Literature DB >> 16503297

Purification and characterization of fenitrothion hydrolase from Burkholderia sp. NF100.

Kanako Tago1, Sumiko Yonezawa, Toshihide Ohkouchi, Masayuki Hashimoto, Masahito Hayatsu.   

Abstract

The organophosphorus pesticide hydrolase was purified to homogeneity from Burkholderia sp. NF100 by detergent extraction of the cell membrane fraction, anion-exchange, chromatofocusing, and gel filtration chromatographies. The purified enzyme had a molecular mass of 55 kDa and a pI 5.8, and the hydrolase activity was strongly inhibited by EDTA, dithiothreitol (DTT), Hg2+ and 1,10-phenanthroline. The optimum pH and temperature for the enzyme activity were 8.0 and 40 degrees C, respectively. The enzyme hydrolyzed five organophosphorus pesticides.

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Year:  2006        PMID： 16503297     DOI： 10.1263/jbb.101.80

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  5 in total

1.  Symbiont-mediated insecticide resistance.

Authors:  Yoshitomo Kikuchi; Masahito Hayatsu; Takahiro Hosokawa; Atsushi Nagayama; Kanako Tago; Takema Fukatsu
Journal:  Proc Natl Acad Sci U S A       Date:  2012-04-23       Impact factor: 11.205

2.  Haloalkylphosphorus hydrolases purified from Sphingomonas sp. strain TDK1 and Sphingobium sp. strain TCM1.

Authors:  Katsumasa Abe; Satoshi Yoshida; Yuto Suzuki; Junichi Mori; Yuka Doi; Shouji Takahashi; Yoshio Kera
Journal:  Appl Environ Microbiol       Date:  2014-07-18       Impact factor: 4.792

3.  Infection dynamics of insecticide-degrading symbionts from soil to insects in response to insecticide spraying.

Authors:  Hideomi Itoh; Tomoyuki Hori; Yuya Sato; Atsushi Nagayama; Kanako Tago; Masahito Hayatsu; Yoshitomo Kikuchi
Journal:  ISME J       Date:  2018-01-17       Impact factor: 10.302

4.  Metagenome Sequencing Reveals the Midgut Microbiota Makeup of Culex pipiens quinquefasciatus and Its Possible Relationship With Insecticide Resistance.

Authors:  Yi-Ting Wang; Rui-Xin Shen; Dan Xing; Chen-Pei Zhao; He-Ting Gao; Jia-Hong Wu; Ning Zhang; Heng-Duan Zhang; Yan Chen; Tong-Yan Zhao; Chun-Xiao Li
Journal:  Front Microbiol       Date:  2021-02-25       Impact factor: 5.640

5.  Seasonal Variation of Midgut Bacterial Diversity in Culexquinquefasciatus Populations in Haikou City, Hainan Province, China.

Authors:  Penghui Suo; Kaixuan Wang; Hongxiao Yu; Xiuhao Fu; Liping An; Biswajit Bhowmick; Jiachao Zhang; Qian Han
Journal:  Biology (Basel)       Date:  2022-08-03
  5 in total

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