| Literature DB >> 16503058 |
Diego Romero1, Alejandro Pérez-García, Jan-Willem Veening, Antonio de Vicente, Oscar P Kuipers.
Abstract
A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods.Entities:
Mesh：
Year: 2006 PMID： 16503058 DOI： 10.1016/j.mimet.2006.01.005
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363