AIMS/HYPOTHESIS: The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes. MATERIALS AND METHODS: Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice. RESULTS: Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity. CONCLUSIONS/ INTERPRETATION: Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes.
AIMS/HYPOTHESIS: The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes. MATERIALS AND METHODS: Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice. RESULTS: Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity. CONCLUSIONS/ INTERPRETATION: Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes.
Authors: Vladimir B Ritov; Elizabeth V Menshikova; Jing He; Robert E Ferrell; Bret H Goodpaster; David E Kelley Journal: Diabetes Date: 2005-01 Impact factor: 9.461
Authors: N Kanemoto; H Hishigaki; A Miyakita; K Oga; S Okuno; A Tsuji; T Takagi; E Takahashi; Y Nakamura; T K Watanabe Journal: Mamm Genome Date: 1998-06 Impact factor: 2.957
Authors: Leanne Wilson-Fritch; Sarah Nicoloro; My Chouinard; Mitchell A Lazar; Patricia C Chui; John Leszyk; Juerg Straubhaar; Michael P Czech; Silvia Corvera Journal: J Clin Invest Date: 2004-11 Impact factor: 14.808
Authors: Kurt Højlund; Krzysztof Wrzesinski; Peter Mose Larsen; Stephen J Fey; Peter Roepstorff; Aase Handberg; Flemming Dela; Jørgen Vinten; James G McCormack; Christine Reynet; Henning Beck-Nielsen Journal: J Biol Chem Date: 2003-01-16 Impact factor: 5.157
Authors: Hana Antonicka; Isla Ogilvie; Tanja Taivassalo; Roberto P Anitori; Ronald G Haller; John Vissing; Nancy G Kennaway; Eric A Shoubridge Journal: J Biol Chem Date: 2003-08-26 Impact factor: 5.157
Authors: Jiandie Lin; Hai Wu; Paul T Tarr; Chen-Yu Zhang; Zhidan Wu; Olivier Boss; Laura F Michael; Pere Puigserver; Eiji Isotani; Eric N Olson; Bradford B Lowell; Rhonda Bassel-Duby; Bruce M Spiegelman Journal: Nature Date: 2002-08-15 Impact factor: 49.962
Authors: T Yamauchi; J Kamon; Y Minokoshi; Y Ito; H Waki; S Uchida; S Yamashita; M Noda; S Kita; K Ueki; K Eto; Y Akanuma; P Froguel; F Foufelle; P Ferre; D Carling; S Kimura; R Nagai; B B Kahn; T Kadowaki Journal: Nat Med Date: 2002-10-07 Impact factor: 53.440
Authors: Luca Vanella; Ming Li; DongHyun Kim; Giuseppe Malfa; Lars Bellner; Tomoko Kawakami; Nader G Abraham Journal: Cell Cycle Date: 2012-02-15 Impact factor: 4.534
Authors: Sandra Kleiner; Rina J Mepani; Dina Laznik; Li Ye; Michael J Jurczak; Francois R Jornayvaz; Jennifer L Estall; Diti Chatterjee Bhowmick; Gerald I Shulman; Bruce M Spiegelman Journal: Proc Natl Acad Sci U S A Date: 2012-05-29 Impact factor: 11.205
Authors: K Højlund; Z Yi; N Lefort; P Langlais; B Bowen; K Levin; H Beck-Nielsen; L J Mandarino Journal: Diabetologia Date: 2009-12-12 Impact factor: 10.122