Alberto Musto1, Nicolas G Bazan. 1. LSU Neuroscience Center of Excellence, Louisiana State University School of Medicine, Health Sciences Center, 2020 Gravier Street, New Orleans, LA 70112, U.S.A.
Abstract
PURPOSE: Diacylglycerol kinase epsilon (DGKepsilon) regulates seizure susceptibility and long-term potentiation through arachidonoyl-inositol lipid signaling. We studied the significance of arachidonoyl-diacylglycerol (20:4 DAG) in epileptogenesis in DGKepsilon-deficient mice undergoing rapid kindling epileptogenesis. METHODS: Tripolar electrode units were implanted in right dorsal hippocampi of male DGKepsilon(+/+) and DGKepsilon(-/-) mice. Ten days after surgery, kindling was achieved by stimulating 6 times daily for 4 days with a subconvulsive electrical stimulation (10-s train of 50-Hz biphasic pulses, 75-200 muA amplitude) at 30-min intervals. After 1 week, mice were rekindled. EEGs were recorded and analyzed to characterize epileptogenic events as spikes, sharp waves, or abnormal amplitudes and rhythms. Right hippocampi were analyzed by histology [Timm's staining, neuropeptide Y (NPY) and glial fibrillary acidic protein immunoreactivity], and for DNA fragmentation (TUNEL). RESULTS: DGKepsilon(-/-) mice had significantly fewer motor seizure and epileptic events compared with DGKepsilon(+/+) mice from the second day of stimulation. These differences were maintained during rekindling. DGKepsilon(-/-) mice also exhibited low-amplitude spike-wave complexes, short spreading depression, and predominant lower-frequency (1-4 Hz) bands throughout stimulation, whereas DGKepsilon(+/+) mice exhibited increased high-frequency bands (4-8 Hz; 8-15 Hz) from the second day of stimulation, as determined by power spectral analysis. DGKepsilon(-/-) mice displayed no sprouting in the supragranular area or NPY inmunoreactivity in the hilus and had weak astrocyte reactivation in all hippocampal areas. No TUNEL-positive cells were detected in any group of mice. CONCLUSIONS: DGKepsilon modulates kindling epileptogenesis through inositol lipid signaling. Because arachidonate-containing diacylglycerol phosphorylation to phosphatidic acid is selectively blocked in DGKepsilon(-/-) mice, we postulate that the shortage of arachidonoyl-moiety inositol lipids and/or the messengers derived thereof is a key signaling event in epileptogenesis.
PURPOSE:Diacylglycerol kinase epsilon (DGKepsilon) regulates seizure susceptibility and long-term potentiation through arachidonoyl-inositol lipid signaling. We studied the significance of arachidonoyl-diacylglycerol (20:4 DAG) in epileptogenesis in DGKepsilon-deficientmice undergoing rapid kindling epileptogenesis. METHODS: Tripolar electrode units were implanted in right dorsal hippocampi of male DGKepsilon(+/+) and DGKepsilon(-/-) mice. Ten days after surgery, kindling was achieved by stimulating 6 times daily for 4 days with a subconvulsive electrical stimulation (10-s train of 50-Hz biphasic pulses, 75-200 muA amplitude) at 30-min intervals. After 1 week, mice were rekindled. EEGs were recorded and analyzed to characterize epileptogenic events as spikes, sharp waves, or abnormal amplitudes and rhythms. Right hippocampi were analyzed by histology [Timm's staining, neuropeptide Y (NPY) and glial fibrillary acidic protein immunoreactivity], and for DNA fragmentation (TUNEL). RESULTS:DGKepsilon(-/-) mice had significantly fewer motor seizure and epileptic events compared with DGKepsilon(+/+) mice from the second day of stimulation. These differences were maintained during rekindling. DGKepsilon(-/-) mice also exhibited low-amplitude spike-wave complexes, short spreading depression, and predominant lower-frequency (1-4 Hz) bands throughout stimulation, whereas DGKepsilon(+/+) mice exhibited increased high-frequency bands (4-8 Hz; 8-15 Hz) from the second day of stimulation, as determined by power spectral analysis. DGKepsilon(-/-) mice displayed no sprouting in the supragranular area or NPY inmunoreactivity in the hilus and had weak astrocyte reactivation in all hippocampal areas. No TUNEL-positive cells were detected in any group of mice. CONCLUSIONS:DGKepsilon modulates kindling epileptogenesis through inositol lipid signaling. Because arachidonate-containing diacylglycerol phosphorylation to phosphatidic acid is selectively blocked in DGKepsilon(-/-) mice, we postulate that the shortage of arachidonoyl-moiety inositol lipids and/or the messengers derived thereof is a key signaling event in epileptogenesis.
Authors: Hana L Goldschmidt; Becky Tu-Sekine; Lenora Volk; Victor Anggono; Richard L Huganir; Daniel M Raben Journal: Cell Rep Date: 2015-12-31 Impact factor: 9.423
Authors: Stephen B Milne; Pavlina T Ivanova; Michelle D Armstrong; David S Myers; Jovana Lubarda; Yulia V Shulga; Matthew K Topham; H Alex Brown; Richard M Epand Journal: Biochemistry Date: 2008-08-15 Impact factor: 3.162