Ursula Frischmann1, Werner Müller. 1. German Research Centre for Biotechnology, Department of Experimental Immunology, Mascheroder Weg 1, D-38124 Braunschweig. urd@gbf.de
Abstract
BACKGROUND: In many cases a frequent monitoring of blood is important, e.g. during an infection. Often the availability of blood is the limiting factor. METHODS: 50 microl blood were stained and analyzed using a standard four-color cytometer. Percentages of leukocytes were calculated by FlowJo software. RESULTS: Our protocol allows the differentiation of B cells, T cells, NK cells, neutrophils, and monocytes/macrophages in small volumes of blood. CONCLUSIONS: Using nine fluorochrome-labeled antibodies and a specific gating strategy we were able to differentiate the main immune cells in minute amounts using one staining step. (c) 2006 International Society for Analytical Cytology.
BACKGROUND: In many cases a frequent monitoring of blood is important, e.g. during an infection. Often the availability of blood is the limiting factor. METHODS: 50 microl blood were stained and analyzed using a standard four-color cytometer. Percentages of leukocytes were calculated by FlowJo software. RESULTS: Our protocol allows the differentiation of B cells, T cells, NK cells, neutrophils, and monocytes/macrophages in small volumes of blood. CONCLUSIONS: Using nine fluorochrome-labeled antibodies and a specific gating strategy we were able to differentiate the main immune cells in minute amounts using one staining step. (c) 2006 International Society for Analytical Cytology.
Authors: Natali Froese; Michael Schwarzer; Ina Niedick; Ursula Frischmann; Mario Köster; Andrea Kröger; Peter P Mueller; Mahtab Nourbakhsh; Bastian Pasche; Jörg Reimann; Peter Staeheli; Hansjörg Hauser Journal: Mol Cell Biol Date: 2006-01 Impact factor: 4.272
Authors: Joel G Turner; Trinayan Kashyap; Jana L Dawson; Juan Gomez; Alexis A Bauer; Steven Grant; Yun Dai; Kenneth H Shain; Mark Meads; Yosef Landesman; Daniel M Sullivan Journal: Oncotarget Date: 2016-11-29