BACKGROUND: To improve monitoring of lung diseases, we analyzed cytokines in exhaled breath condensate (EBC). The main challenge in measurement of cytokines in EBC is the low protein content, which requires concentration steps that conflict with the need for excessive fluid required by most commonly used kits. METHODS: Here, a multiplex bead array for the detection of interleukins (IL) -1beta, -6, -8, -10, TNF-alpha, and IL-12p70 was modified and validated for analysis in EBC samples. Furthermore, 33 healthy volunteers and 11 patients with acute lung injury were investigated. RESULTS: In patients with inflammatory lung diseases, cytokine levels for all investigated cytokines were higher in comparison to healthy smokers or healthy volunteers. DISCUSSION: Multiplexed immunoassays in highly sensitive approaches allow for cytokine detection in EBC. We found significant differences between patients and controls for all investigated cytokines. (c) 2005 International Society for Analytical Cytology.
BACKGROUND: To improve monitoring of lung diseases, we analyzed cytokines in exhaled breath condensate (EBC). The main challenge in measurement of cytokines in EBC is the low protein content, which requires concentration steps that conflict with the need for excessive fluid required by most commonly used kits. METHODS: Here, a multiplex bead array for the detection of interleukins (IL) -1beta, -6, -8, -10, TNF-alpha, and IL-12p70 was modified and validated for analysis in EBC samples. Furthermore, 33 healthy volunteers and 11 patients with acute lung injury were investigated. RESULTS: In patients with inflammatory lung diseases, cytokine levels for all investigated cytokines were higher in comparison to healthy smokers or healthy volunteers. DISCUSSION: Multiplexed immunoassays in highly sensitive approaches allow for cytokine detection in EBC. We found significant differences between patients and controls for all investigated cytokines. (c) 2005 International Society for Analytical Cytology.
Authors: T Ichikawa; K Matsunaga; Y Minakata; S Yanagisawa; K Ueshima; K Akamatsu; T Hirano; M Nakanishi; H Sugiura; T Yamagata; M Ichinose Journal: Anal Chem Insights Date: 2007-10-12
Authors: Juan M Diez-Pina; María J Fernandez-Aceñero; María J Llorente-Alonso; Salvador Diaz-Lobato; Sagrario Mayoralas; Asuncion Florez Journal: Int J Gen Med Date: 2009-07-30
Authors: S T Schmid; J Koepke; M Dresel; A Hattesohl; E Frenzel; J Perez; D A Lomas; E Miranda; T Greulich; S Noeske; M Wencker; H Teschler; C Vogelmeier; S Janciauskiene; A R Koczulla Journal: Int J Chron Obstruct Pulmon Dis Date: 2012-09-28