Literature DB >> 1649427

A cloned kinetoplast DNA mini-circle fragment from a Leishmania spp. specific for post-kala-azar dermal leishmaniasis strains.

S Das Gupta1, D K Ghosh, H K Majumder.   

Abstract

DNA-DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis.

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Year:  1991        PMID： 1649427     DOI： 10.1017/s0031182000062478

Source DB:  PubMed          Journal:  Parasitology        ISSN： 0031-1820            Impact factor:   3.234


  9 in total

1.  Chromatographic separation of DNA dependent RNA polymerases and molecular properties of RNA polymerase II from a Leishmania Spp.

Authors:  P K Sadhukhan; A K Chakraborty; A Dasgupta; H K Majumder
Journal:  Mol Cell Biochem       Date:  1997-06       Impact factor: 3.396

2.  Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen.

Authors:  P Salotra; G Sreenivas; A A Nasim; B V Subba Raju; V Ramesh
Journal:  Clin Diagn Lab Immunol       Date:  2002-03

Review 3.  Species typing in dermal leishmaniasis.

Authors:  Gert Van der Auwera; Jean-Claude Dujardin
Journal:  Clin Microbiol Rev       Date:  2015-04       Impact factor: 26.132

Review 4.  DNA probes and PCR for diagnosis of parasitic infections.

Authors:  J B Weiss
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

5.  DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans.

Authors:  Gannavaram Sreenivas; B V Subba Raju; Ruchi Singh; Angamuthu Selvapandiyan; Robert Duncan; Dwijen Sarkar; Hira L Nakhasi; Poonam Salotra
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

Review 6.  Leishmaniases of the New World: current concepts and implications for future research.

Authors:  G Grimaldi; R B Tesh
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

Review 7.  Recent developments leading toward a paradigm switch in the diagnostic and therapeutic approach to human leishmaniasis.

Authors:  Albert Schriefer; Mary E Wilson; Edgar M Carvalho
Journal:  Curr Opin Infect Dis       Date:  2008-10       Impact factor: 4.915

8.  Protective and pathologic immune responses in human tegumentary leishmaniasis.

Authors:  Lucas P Carvalho; Sara Passos; Albert Schriefer; Edgar M Carvalho
Journal:  Front Immunol       Date:  2012-10-04       Impact factor: 7.561

9.  Analysis of genetic polymorphisms and tropism in East African Leishmania donovani by Amplified Fragment Length Polymorphism and kDNA minicircle sequencing.

Authors:  Hanan T Jaber; Asrat Hailu; Francine Pratlong; Patrick Lami; Patrick Bastien; Charles L Jaffe
Journal:  Infect Genet Evol       Date:  2018-07-19       Impact factor: 3.342
  9 in total

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