Literature DB >> 164935

Application of affinity chromatography to the purification of collagenase for the isolation of insoluble elastin.

A Serafini-Fracassini, J M Field, G W Rodger, M Spina.   

Abstract

ClostrIdium histolyticum collagenase (clostridiopeptidase A, EC 3.4.4.19) was purified by batchwise separation with DEAE-cellulose followed by affinity chromatography on a column of alkali-treated elastin. The N-terminal amino acid profile of elastin isolated from bovine ligamentum nuchae using this enzyme preparation was compared with that of a duplicate sample purified with a mixture of collagenase I and II (Yoshida, E, and Noda, H. (1965) Biochim. Biopsys. Acta 105, 562-574). An approx. three-fold decrease in the molar concentration of N-terminal residues and a considerable reduction in their number was obtained by using the former enzyme preparation.

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Year:  1975        PMID： 164935     DOI： 10.1016/0005-2795(75)90248-2

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  5 in total

1.  Proceedings: Pinna reflex activated gamma-efferents in the conduction velocity spectrum to hind-limb muscles in the rat.

Authors:  B L Andrew; G C Leslie; N J Part
Journal:  J Physiol       Date:  1975-09       Impact factor: 5.182

2.  Observations on the different substrate behavior of tropocollagen molecules in solution and intermolecularly cross-linked tropocollagen within insoluble polymeric collagen fibrils.

Authors:  F S Steven
Journal:  Biochem J       Date:  1976-05-01       Impact factor: 3.857

3.  A compilation of amino acid analyses of proteins. XVIII. Residues per thousand residues--5.

Authors:  D M Kirschenbaum
Journal:  Appl Biochem Biotechnol       Date:  1983-08       Impact factor: 2.926

4.  Marfan syndrome. Demonstration of abnormal elastin in aorta.

Authors:  P A Abraham; A J Perejda; W H Carnes; J Uitto
Journal:  J Clin Invest       Date:  1982-12       Impact factor: 14.808

5.  Biomechanical and biochemical properties of the thoracic aorta in warmblood horses, Friesian horses, and Friesians with aortic rupture.

Authors:  Veronique Saey; Nele Famaey; Marija Smoljkic; Erik Claeys; Gunther van Loon; Richard Ducatelle; Margreet Ploeg; Catherine Delesalle; Andrea Gröne; Luc Duchateau; Koen Chiers
Journal:  BMC Vet Res       Date:  2015-11-18       Impact factor: 2.741
  5 in total

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