| Literature DB >> 16490289 |
Lisset Hermida1, Lídice Bernardo, Jorge Martín, Mayling Alvarez, Irina Prado, Carlos López, Beatriz de la C Sierra, Rafael Martínez, Rosmary Rodríguez, Aída Zulueta, Ana B Pérez, Laura Lazo, Delfina Rosario, Gerardo Guillén, María G Guzmán.
Abstract
We have previously reported the construction and evaluation in mice of recombinant fusion proteins formed by a fragment (aa 286-426) of the dengue envelope protein and the P64k protein from Neisseria meningitidis. In this work we describe the immunization of Macaca fascicularis monkeys with two variants of these proteins [PD3 (insertion variant) and PD5 (fusion variant)] corresponding to serotype 2. Four doses of the proteins adjuvated in Freund's adjuvant were administered and the kinetics of antibody induction was monitored by ELISA and neutralization tests. Monkeys receiving PD3 or PD5 developed functional antibodies (Abs) in a dose-dependent manner. Following challenge with 5 log PFU of wild type dengue-2 virus (DEN2), animals immunized with PD5 were protected from developing viremia. These results constitute a proof-of-concept demonstrating that a fragment of the dengue envelope protein, containing the domain III and produced as a recombinant fusion protein in Escherichia coli, induces functional and protective immunity in a nonhuman primate model.Entities:
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Year: 2006 PMID： 16490289 DOI： 10.1016/j.vaccine.2006.01.036
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641