Literature DB >> 1648898

Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose.

J Bustos1, P Zamora, E Mejía, Y Varela, B Gómez.   

Abstract

Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

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Year:  1991        PMID： 1648898     DOI： 10.1007/bf01314039

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  9 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Quantitation of rubella virus by competitive enzyme immunosorbent assay.

Authors:  Y Varela; E Ortega; B Gómez
Journal:  J Virol Methods       Date:  1988-01       Impact factor: 2.014

3.  Equine abortion (herpes) virus: purification and concentration of enveloped and deenveloped virus and envelope material by density gradient centrifugation in colloidal silica.

Authors:  B Klingeborn; H Pertoft
Journal:  Virology       Date:  1972-05       Impact factor: 3.616

4.  Density gradient centrifugation of a herpesvirus (IBRV) in colloidal silica.

Authors:  H Pertoft
Journal:  Virology       Date:  1970-06       Impact factor: 3.616

5.  Density gradient ultracentrifugation of rubella virus.

Authors:  M S Amstey; T E Hobbins; P D Parkman
Journal:  Proc Soc Exp Biol Med       Date:  1968-04

6.  The purification of rubella virus (RV) and determination of its polypeptide composition.

Authors:  D Van Alstyne; G Krystal; G D Kettyls; E M Bohn
Journal:  Virology       Date:  1981-01-30       Impact factor: 3.616

7.  A method for quantitation of protein in the presence of Percoll.

Authors:  M N Khan; R J Khan; B I Posner
Journal:  Anal Biochem       Date:  1981-10       Impact factor: 3.365

8.  Concentration and purification of rubella virus hemagglutinin by hollow fiber ultrafiltration and sucrose density centrifugation.

Authors:  M Trudel; P Payment
Journal:  Can J Microbiol       Date:  1980-11       Impact factor: 2.419

9.  Purification of rubella virus particles.

Authors:  A Vaheri; C H von Bonsdorff; T Vesikari; T Hovi; P Väänänen
Journal:  J Gen Virol       Date:  1969-07       Impact factor: 3.891
  9 in total

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