| Literature DB >> 16487631 |
Sandra Romero-Steiner1, Joseph Caba, Gowrisankar Rajam, Travis Langley, Andrea Floyd, Scott E Johnson, Jacquelyne S Sampson, George M Carlone, Edwin Ades.
Abstract
Pneumococcal surface adhesin A (PsaA) is a pneumococcal vaccine candidate. In this study, we detect functional antibodies to PsaA by using carboxylate-modified fluospheres coated with either recombinant non-lipidated PsaA (rPsaA) or synthetic peptides with relevant epitopes of PsaA. Peptides P1-P3 were derived from phage display sequences; peptides P4-P7 were homologous to rPsaA. P1- and P4-coated fluospheres had similar adherence to Detroit 562 nasopharyngeal cells when compared to rPsaA-coated fluospheres. Homologous and heterologous competitive inhibitions with peptides in solution determined the specificity of the adherence. There was no significant difference (P=0.25) between the inhibition of adherence of rPsaA- and P4-coated fluospheres. This study indicates that P1 and P4 contain a functional epitope(s) for the adherence of PsaA to nasopharyngeal cells making them suitable targets for the measurement of functional antibodies to PsaA.Entities:
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Year: 2006 PMID： 16487631 DOI： 10.1016/j.vaccine.2006.01.042
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641