| Literature DB >> 16483820 |
Cinzia Marianelli1, Franco Ciuchini, Michela Tarantino, Paolo Pasquali, Rosanna Adone.
Abstract
The rpoB gene encoding the beta subunit of the DNA-dependent RNA polymerase was molecularly characterized by PCR amplification and DNA sequencing in 26 Brucella reference strains by using primers selected according to the B. melitensis 16 M rpoB published sequence. Comparison of the rpoB nucleotide sequence of all Brucella strains analysed revealed specific nucleotide variations associated with different Brucella species and biovars. 17 rpoB alleles were recognized and new Brucella typing is proposed. Our results suggest that the rpoB gene polymorphism can be used to identify all Brucella species and most of the biovars, offering an improvement over conventional typing methods.Entities:
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Year: 2006 PMID： 16483820 DOI： 10.1016/j.micinf.2005.10.008
Source DB: PubMed Journal: Microbes Infect ISSN： 1286-4579 Impact factor: 2.700