Qing Yao1, Xiao-song He, Jian-min Zhang, Jie He. 1. Department of Pathology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu, 210009, China. njyqfm@sohu.com
Abstract
OBJECTIVE: To investigate promotor hypermethylation of the CpG islands of E-Cadherin, p16 and estrogen receptor (ER) in prostate carcinoma and explore whether such methylation might play a role in the tumorigenesis and malignant progression of prostate carcinoma and their significance in the diagnosis, prognosis and treatment. METHODS: Thirteen specimens of benign prostatic hypertrophy (BPH), 10 specimens of high grade prostate intraepithelial neoplasia (HGPIN) and 20 specimens of prostate carcinoma (with follow-up records) were collected from the patients who underwent radical prostatectomy or transurethral resection. Methylation-specific PCR (MSP) was used to detect the promoter hypermethylation of E-Cadherin, p16 and ER genes. RESULTS: Hypermethylation percentages in prostate carcinoma were: E-Cadherin, 30%; p16, 25%; and ER, 65%. Almost all nonmalignant tissues (BPH and HG-PIN) lacked methylation of any genes. CONCLUSION: Detecting promotor hypermethylation of E-Cadherin, p16 and ER may be helpful to differentiate nonmalignant lesions from prostate carcinoma.
OBJECTIVE: To investigate promotor hypermethylation of the CpG islands of E-Cadherin, p16 and estrogen receptor (ER) in prostate carcinoma and explore whether such methylation might play a role in the tumorigenesis and malignant progression of prostate carcinoma and their significance in the diagnosis, prognosis and treatment. METHODS: Thirteen specimens of benign prostatic hypertrophy (BPH), 10 specimens of high grade prostate intraepithelial neoplasia (HGPIN) and 20 specimens of prostate carcinoma (with follow-up records) were collected from the patients who underwent radical prostatectomy or transurethral resection. Methylation-specific PCR (MSP) was used to detect the promoter hypermethylation of E-Cadherin, p16 and ER genes. RESULTS: Hypermethylation percentages in prostate carcinoma were: E-Cadherin, 30%; p16, 25%; and ER, 65%. Almost all nonmalignant tissues (BPH and HG-PIN) lacked methylation of any genes. CONCLUSION: Detecting promotor hypermethylation of E-Cadherin, p16 and ER may be helpful to differentiate nonmalignant lesions from prostate carcinoma.
Authors: Ahmad Ameri; Asdollah Alidoosti; Seyed Yousef Hosseini; Mohammad Parvin; Mohammad Hasan Emranpour; Farnaz Taslimi; Eisa Salehi; Pedram Fadavip Journal: Chin J Cancer Res Date: 2011-12 Impact factor: 5.087