BACKGROUND: In tissue context, researchers and pathologists lack a generally applicable standard for quantitative determination of cytological parameters. Increasing knowledge of disease-specific markers calls for an appropriate in situ tissue cytometry. METHODS: Microscopy-based multicolor tissue cytometry (MMTC) permits multicolor analysis of single cells within tissue context. RESULTS: Tissue specimens stained for CD45/CD3/CD4/CD8 were analyzed. Specificity as well as reproducibility of MMTC is demonstrated and a novel MMTC-based function to improve visual discrimination of subpopulations is introduced. CONCLUSIONS: Our data demonstrate that MMTC constitutes an important step toward automated and quantitative fluorometry of solid tissues and cell monolayers. (c) 2006 International Society for Analytical Cytology.
BACKGROUND: In tissue context, researchers and pathologists lack a generally applicable standard for quantitative determination of cytological parameters. Increasing knowledge of disease-specific markers calls for an appropriate in situ tissue cytometry. METHODS: Microscopy-based multicolor tissue cytometry (MMTC) permits multicolor analysis of single cells within tissue context. RESULTS: Tissue specimens stained for CD45/CD3/CD4/CD8 were analyzed. Specificity as well as reproducibility of MMTC is demonstrated and a novel MMTC-based function to improve visual discrimination of subpopulations is introduced. CONCLUSIONS: Our data demonstrate that MMTC constitutes an important step toward automated and quantitative fluorometry of solid tissues and cell monolayers. (c) 2006 International Society for Analytical Cytology.
Authors: Seth Winfree; Michael J Ferkowicz; Pierre C Dagher; Katherine J Kelly; Michael T Eadon; Timothy A Sutton; Troy A Markel; Mervin C Yoder; Kenneth W Dunn; Tarek M El-Achkar Journal: Transl Res Date: 2017-07-22 Impact factor: 7.012
Authors: Jun Wei; Hongyan Zhu; Gabriel Lord; Mitra Bhattachayya; Brielle M Jones; Graham Allaway; Shyam S Biswal; Benjamin Korman; Roberta G Marangoni; Warren G Tourtellotte; John Varga Journal: Transl Res Date: 2016-12-09 Impact factor: 7.012
Authors: Arthur Liesz; Li Sun; Wei Zhou; Sönke Schwarting; Eva Mracsko; Markus Zorn; Henrike Bauer; Clemens Sommer; Roland Veltkamp Journal: PLoS One Date: 2011-06-20 Impact factor: 3.240
Authors: Claudia Chavez-Munoz; Khang T Nguyen; Wei Xu; Seok-Jong Hong; Thomas A Mustoe; Robert D Galiano Journal: PLoS One Date: 2013-12-02 Impact factor: 3.240