Literature DB >> 16473594

Purification and analysis of RIN family-novel Rab5 GEFs.

Kota Saito, Hiroaki Kajiho, Yasuhiro Araki, Hiroshi Kurosu, Kenji Kontani, Hiroshi Nishina, Toshiaki Katada.   

Abstract

The small GTPase Rab5 plays important roles in membrane budding and trafficking in the early endocytic pathways, and the activation of this GTPase is mediated by several guanine nucleotide exchange factors (GEFs) at each of the transport steps. The RIN family has been identified as GEFs for Rab5 and shown to possess unique biochemical properties. The RIN family preferentially interacts with an activated form of Rab5, although it enhances guanine nucleotide exchange reaction. Moreover, biochemical analysis indicates that the RIN family functions as a tetramer. In this chapter, we describe the isolation of the recombinant RIN family via expression in Spodoptera frugiperda (Sf9) insect cells and in mammalian cells. In addition, functional analysis is also provided to assess the physiological properties of the RIN family.

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Year:  2005        PMID： 16473594     DOI： 10.1016/S0076-6879(05)03023-5

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  6 in total

1.  Overexpression of RIN1 associates with tumor grade and progression in patients of bladder urothelial carcinoma.

Authors:  Guang-yi Shan; Zhe Zhang; Qi-guang Chen; Xiu-yue Yu; Guo-bin Liu; Chui-ze Kong
Journal:  Tumour Biol       Date:  2012-01-17

2.  Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31.

Authors:  Hiroaki Kajiho; Kyoko Sakurai; Tomohiro Minoda; Manabu Yoshikawa; Satoshi Nakagawa; Shinichi Fukushima; Kenji Kontani; Toshiaki Katada
Journal:  J Biol Chem       Date:  2011-05-17       Impact factor: 5.157

3.  Altered oligomeric states in pathogenic ALS2 variants associated with juvenile motor neuron diseases cause loss of ALS2-mediated endosomal function.

Authors:  Kai Sato; Asako Otomo; Mahoko Takahashi Ueda; Yui Hiratsuka; Kyoko Suzuki-Utsunomiya; Junya Sugiyama; Shuji Murakoshi; Shun Mitsui; Suzuka Ono; So Nakagawa; Hui-Fang Shang; Shinji Hadano
Journal:  J Biol Chem       Date:  2018-09-17       Impact factor: 5.157

4.  The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and morphogenesis via active integrin endocytosis and Rac signaling.

Authors:  Chiara Sandri; Francesca Caccavari; Donatella Valdembri; Chiara Camillo; Stefan Veltel; Martina Santambrogio; Letizia Lanzetti; Federico Bussolino; Johanna Ivaska; Guido Serini
Journal:  Cell Res       Date:  2012-07-24       Impact factor: 25.617

5.  RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin.

Authors:  Hiroaki Kajiho; Shinichi Fukushima; Kenji Kontani; Toshiaki Katada
Journal:  PLoS One       Date:  2012-01-23       Impact factor: 3.240

6.  Concentration of Sec12 at ER exit sites via interaction with cTAGE5 is required for collagen export.

Authors:  Kota Saito; Koh Yamashiro; Noriko Shimazu; Tomoya Tanabe; Kenji Kontani; Toshiaki Katada
Journal:  J Cell Biol       Date:  2014-09-08       Impact factor: 10.539
  6 in total

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