Literature DB >> 16473591

Fluorescent microscopy-based assays to study the role of Rab22a in clathrin-independent endocytosis.

Roberto Weigert, Julie G Donaldson.   

Abstract

The endocytic and trafficking route followed by proteins that enter cells independently of clathrin is not well understood. In HeLa cells, a distinct endocytic and recycling pathway is followed for such clathrin-independent cargo proteins, and we have characterized the role of Rab proteins in this process. Here we describe cell-based flourescence assays to examine the effects of expression and depletion of Rab22 on endosomal morphology and endocytic recycling.

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Year:  2005        PMID： 16473591     DOI： 10.1016/S0076-6879(05)03020-X

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  7 in total

1.  Regulation of cell death by recycling endosomes and golgi membrane dynamics via a pathway involving Src-family kinases, Cdc42 and Rab11a.

Authors:  Marie-Claude Landry; Andréane Sicotte; Claudia Champagne; Josée N Lavoie
Journal:  Mol Biol Cell       Date:  2009-07-29       Impact factor: 4.138

2.  Determining the macropinocytic index of cells through a quantitative image-based assay.

Authors:  Cosimo Commisso; Rory J Flinn; Dafna Bar-Sagi
Journal:  Nat Protoc       Date:  2014-01-02       Impact factor: 13.491

3.  A novel requirement for C. elegans Alix/ALX-1 in RME-1-mediated membrane transport.

Authors:  Anbing Shi; Saumya Pant; Zita Balklava; Carlos Chih-Hsiung Chen; Vanesa Figueroa; Barth D Grant
Journal:  Curr Biol       Date:  2007-11-08       Impact factor: 10.834

4.  A role for EHD4 in the regulation of early endosomal transport.

Authors:  Mahak Sharma; Naava Naslavsky; Steve Caplan
Journal:  Traffic       Date:  2008-03-06       Impact factor: 6.215

5.  Host factor Rab11a is critical for efficient assembly of influenza A virus genomic segments.

Authors:  Julianna Han; Ketaki Ganti; Veeresh Kumar Sali; Carly Twigg; Yifeng Zhang; Senthamizharasi Manivasagam; Chieh-Yu Liang; Olivia A Vogel; Iris Huang; Shanan N Emmanuel; Jesse Plung; Lillianna Radoshevich; Jasmine T Perez; Anice C Lowen; Balaji Manicassamy
Journal:  PLoS Pathog       Date:  2021-05-10       Impact factor: 6.823

6.  TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanism.

Authors:  Archana Varadaraj; Laura M Jenkins; Priyanka Singh; Anindya Chanda; John Snider; N Y Lee; Ayelet R Amsalem-Zafran; Marcelo Ehrlich; Yoav I Henis; Karthikeyan Mythreye
Journal:  Mol Biol Cell       Date:  2017-03-15       Impact factor: 4.138

7.  Trafficking of the Menkes copper transporter ATP7A is regulated by clathrin-, AP-2-, AP-1-, and Rab22-dependent steps.

Authors:  Zoe G Holloway; Antonio Velayos-Baeza; Gareth J Howell; Clotilde Levecque; Sreenivasan Ponnambalam; Elizabeth Sztul; Anthony P Monaco
Journal:  Mol Biol Cell       Date:  2013-04-17       Impact factor: 4.138
  7 in total

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