PURPOSE: To evaluate the protective effects of antioxidant agents against space radiation-induced oxidative stress in cultured human epithelial cells. METHODS AND MATERIALS: The effects of selected concentrations of N-acetylcysteine, ascorbic acid, sodium ascorbate, co-enzyme Q10, alpha-lipoic acid, l-selenomethionine, and vitamin E succinate on radiation-induced oxidative stress were evaluated in MCF10 human breast epithelial cells exposed to radiation with X-rays, gamma-rays, protons, or high mass, high atomic number, and high energy particles using a dichlorofluorescein assay. RESULTS: The results demonstrated that these antioxidants are effective in protecting against radiation-induced oxidative stress and complete or nearly complete protection was achieved by treating the cells with a combination of these agents before and during the radiation exposure. CONCLUSION: The combination of antioxidants evaluated in this study is likely be a promising countermeasure for protection against space radiation-induced adverse biologic effects.
PURPOSE: To evaluate the protective effects of antioxidant agents against space radiation-induced oxidative stress in cultured human epithelial cells. METHODS AND MATERIALS: The effects of selected concentrations of N-acetylcysteine, ascorbic acid, sodium ascorbate, co-enzyme Q10, alpha-lipoic acid, l-selenomethionine, and vitamin E succinate on radiation-induced oxidative stress were evaluated in MCF10 human breast epithelial cells exposed to radiation with X-rays, gamma-rays, protons, or high mass, high atomic number, and high energy particles using a dichlorofluorescein assay. RESULTS: The results demonstrated that these antioxidants are effective in protecting against radiation-induced oxidative stress and complete or nearly complete protection was achieved by treating the cells with a combination of these agents before and during the radiation exposure. CONCLUSION: The combination of antioxidants evaluated in this study is likely be a promising countermeasure for protection against space radiation-induced adverse biologic effects.
Authors: Chris Wambi; Jenine Sanzari; X Steven Wan; Manunya Nuth; James Davis; Ying-Hui Ko; Carly M Sayers; Matthew Baran; Jeffrey H Ware; Ann R Kennedy Journal: Radiat Res Date: 2008-04 Impact factor: 2.841
Authors: Julianne M Pollard; Julio S Reboucas; Armando Durazo; Ivan Kos; Francesca Fike; Moeen Panni; Edith Butler Gralla; Joan Selverstone Valentine; Ines Batinic-Haberle; Richard A Gatti Journal: Free Radic Biol Med Date: 2009-04-21 Impact factor: 7.376
Authors: Minghong Li; Veronica Holmes; Houping Ni; Jenine K Sanzari; Ana L Romero-Weaver; Liyong Lin; Alejandro Carabe-Fernandez; Eric S Diffenderfer; Ann R Kennedy; Drew Weissman Journal: PLoS One Date: 2015-03-20 Impact factor: 3.240