| Literature DB >> 16472875 |
Sonja Wilhelm1, Pia Zimmermann, Hans Joachim Selbitz, Uwe Truyen.
Abstract
This report describes a real-time polymerase chain reaction assay with SYBR Green for detection of a broad range of porcine parvoviruses (PPV) and accurate virus quantification in porcine tissues. The assay targets the VP2 gene of PPV and the porcine genomic c-myc gene for normalization. The detection limit of the SYBR Green reaction was shown to be equivalent to 6 x 10(0) to 6 x 10(1) PPV copies/reaction and the overall detection limit equivalent to 0.1 TCID(50)/100 microl. The assay was linear over a 10(7) dilution range of template concentrations. Other porcine pathogens involved in reproductive disorders such as porcine circovirus 2 (PCV-2), porcine reproductive and respiratory virus (PRRSV), Aujeszky's disease virus (PRV) and other parvoviruses such as feline parvovirus (FPV), canine parvovirus (CPV), minute virus of canines (MVC) and a human parvovirus (B19) were not detected by this assay.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16472875 DOI： 10.1016/j.jviromet.2006.01.004
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014