| Literature DB >> 16472858 |
Trevor J McFarland1, Yi Zhang, La-ongsri Atchaneeyaskul, Peter Francis, J Timothy Stout, Binoy Appukuttan.
Abstract
The soluble neuropilin-1 (sNRP-1) gene employs an extremely short dual function polyadenylation (pA) signal/stop codon that is efficient for termination of gene transcription and translation in vivo. However, the functionality and usefulness of this signal in regard to other genes is unknown. This quantitative study compares the levels of humanized Renilla green fluorescent protein (hrGFP) mRNA polyadenylated with either the sNRP-1 pA signal or the much larger, more widely used SV40 pA signal. We show that the overall starting copy number of hrGFP for equally loaded RNAs is equivalent between the two groups. Our data show little to no difference between levels of mRNA generated by the sNRP-1 polyadenylation signal and the SV40 polyadenylation signal despite a remarkable size difference in signal length. An extremely short polyadenylation signal could potentially alleviate gene insert size restrictions associated with cloning or with therapeutic vectors such as adeno-associated virus.Entities:
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Year: 2006 PMID： 16472858 DOI： 10.1016/j.plasmid.2005.11.005
Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466