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Literature DB >> 16472689

Real-time centrosome reorientation during fibroblast migration.

Abstract

The centrosome is positioned between the nucleus and the leading edge of many types of migrating cells. Cdc42 regulates this centrosome reorientation through its effectors Par6 and MRCK. Using time-lapse microscopy of live cells, the mechanisms and kinetics of centrosome reorientation can be studied. In this chapter, we describe a modification in the standard wound healing assay that allows the study of signaling pathways involved in centrosome reorientation and other polarization events that occur before cell migration. We also describe a method for visualization of centrosome reorientation by time-lapse microscopy using NIH 3T3 fibroblasts stably transfected with GFP-tubulin.

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Year: 2006 PMID： 16472689 DOI： 10.1016/S0076-6879(06)06045-9

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

15 in total

1. Linker of nucleoskeleton and cytoskeleton (LINC) complex-mediated actin-dependent nuclear positioning orients centrosomes in migrating myoblasts.

Authors: Wakam Chang; Susumu Antoku; Cecilia Östlund; Howard J Worman; Gregg G Gundersen
Journal: Nucleus Date: 2015 Impact factor: 4.197

2. FAK, talin and PIPKIγ regulate endocytosed integrin activation to polarize focal adhesion assembly.

Authors: Guilherme P F Nader; Ellen J Ezratty; Gregg G Gundersen
Journal: Nat Cell Biol Date: 2016-04-04 Impact factor: 28.824

3. Lamin A variants that cause striated muscle disease are defective in anchoring transmembrane actin-associated nuclear lines for nuclear movement.

Authors: Eric S Folker; Cecilia Ostlund; G W Gant Luxton; Howard J Worman; Gregg G Gundersen
Journal: Proc Natl Acad Sci U S A Date: 2010-12-20 Impact factor: 11.205

Real-time centrosome reorientation during fibroblast migration.

1. Linker of nucleoskeleton and cytoskeleton (LINC) complex-mediated actin-dependent nuclear positioning orients centrosomes in migrating myoblasts.

2. FAK, talin and PIPKIγ regulate endocytosed integrin activation to polarize focal adhesion assembly.

3. Lamin A variants that cause striated muscle disease are defective in anchoring transmembrane actin-associated nuclear lines for nuclear movement.

Review 4. Fibrous stroma: Driver and passenger in cancer development.

5. Dynein drives nuclear rotation during forward progression of motile fibroblasts.

6. Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia.

7. Centrifugal Displacement of Nuclei Reveals Multiple LINC Complex Mechanisms for Homeostatic Nuclear Positioning.

8. Par3 and dynein associate to regulate local microtubule dynamics and centrosome orientation during migration.

9. Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells.

10. TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton.