Literature DB >> 16472675

Analysis of activated GAPs and GEFs in cell lysates.

Rafael García-Mata1, Krister Wennerberg, William T Arthur, Nicole K Noren, Shawn M Ellerbroek, Keith Burridge.   

Abstract

An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals.

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Year:  2006        PMID： 16472675     DOI： 10.1016/S0076-6879(06)06031-9

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  126 in total

1.  Polarity-regulating kinase partitioning-defective 1b (PAR1b) phosphorylates guanine nucleotide exchange factor H1 (GEF-H1) to regulate RhoA-dependent actin cytoskeletal reorganization.

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Journal:  J Biol Chem       Date:  2011-11-09       Impact factor: 5.157

2.  Extracellular signal-regulated kinase and GEF-H1 mediate depolarization-induced Rho activation and paracellular permeability increase.

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Journal:  Am J Physiol Cell Physiol       Date:  2010-03-17       Impact factor: 4.249

3.  Mitotic-dependent phosphorylation of leukemia-associated RhoGEF (LARG) by Cdk1.

Authors:  Michelle C Helms; Elda Grabocka; Matthew K Martz; Christopher C Fischer; Nobuchika Suzuki; Philip B Wedegaertner
Journal:  Cell Signal       Date:  2015-10-19       Impact factor: 4.315

4.  Staphylococcus aureus-induced endothelial permeability and inflammation are mediated by microtubule destabilization.

Authors:  Pratap Karki; Yunbo Ke; Yufeng Tian; Tomomi Ohmura; Albert Sitikov; Nicolene Sarich; Christopher P Montgomery; Anna A Birukova
Journal:  J Biol Chem       Date:  2019-01-08       Impact factor: 5.157

5.  Drosophila Syd-1 Has RhoGAP Activity That Is Required for Presynaptic Clustering of Bruchpilot/ELKS but Not Neurexin-1.

Authors:  Michael A Spinner; David A Walla; Tory G Herman
Journal:  Genetics       Date:  2017-12-07       Impact factor: 4.562

6.  The RhoA guanine nucleotide exchange factor, LARG, mediates ICAM-1-dependent mechanotransduction in endothelial cells to stimulate transendothelial migration.

Authors:  Elizabeth C Lessey-Morillon; Lukas D Osborne; Elizabeth Monaghan-Benson; Christophe Guilluy; E Timothy O'Brien; Richard Superfine; Keith Burridge
Journal:  J Immunol       Date:  2014-02-28       Impact factor: 5.422

7.  Hepatocyte growth factor-induced Asef-IQGAP1 complex controls cytoskeletal remodeling and endothelial barrier.

Authors:  Yufeng Tian; Grzegorz Gawlak; Alok S Shah; Katherine Higginbotham; Xinyong Tian; Yoshihiro Kawasaki; Tetsu Akiyama; David B Sacks; Anna A Birukova
Journal:  J Biol Chem       Date:  2014-12-09       Impact factor: 5.157

8.  Antibody Binding to CD4 Induces Rac GTPase Activation and Alters T Cell Migration.

Authors:  Y Maurice Morillon; Elizabeth Chase Lessey-Morillon; Matthew Clark; Rui Zhang; Bo Wang; Keith Burridge; Roland Tisch
Journal:  J Immunol       Date:  2016-09-30       Impact factor: 5.422

9.  p66Shc Couples Mechanical Signals to RhoA through Focal Adhesion Kinase-Dependent Recruitment of p115-RhoGEF and GEF-H1.

Authors:  Ru-Feng Wu; Chengxu Liao; Guosheng Fu; Heather N Hayenga; Kejia Yang; Zhenyi Ma; Zhe Liu; Lance S Terada
Journal:  Mol Cell Biol       Date:  2016-10-28       Impact factor: 4.272

10.  Variegated RHOA mutations in adult T-cell leukemia/lymphoma.

Authors:  Yasunobu Nagata; Kenji Kontani; Terukazu Enami; Keisuke Kataoka; Ryohei Ishii; Yasushi Totoki; Tatsuki R Kataoka; Masahiro Hirata; Kazuhiro Aoki; Kazumi Nakano; Akira Kitanaka; Mamiko Sakata-Yanagimoto; Sachiko Egami; Yuichi Shiraishi; Kenichi Chiba; Hiroko Tanaka; Yusuke Shiozawa; Tetsuichi Yoshizato; Hiromichi Suzuki; Ayana Kon; Kenichi Yoshida; Yusuke Sato; Aiko Sato-Otsubo; Masashi Sanada; Wataru Munakata; Hiromi Nakamura; Natsuko Hama; Satoru Miyano; Osamu Nureki; Tatsuhiro Shibata; Hironori Haga; Kazuya Shimoda; Toshiaki Katada; Shigeru Chiba; Toshiki Watanabe; Seishi Ogawa
Journal:  Blood       Date:  2015-11-16       Impact factor: 22.113
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