Literature DB >> 16472661

Purification and kinase assay of PKN.

Hideyuki Mukai1, Yoshitaka Ono.   

Abstract

PKN is a serine/threonine protein kinase, which has a catalytic domain highly homologous to that of protein kinase C (PKC) in the carboxyl-terminal region and three repeats of the antiparallel coiled coil (ACC) domain in the amino-terminal region. Mammalian PKN has three isoforms each derived from different genes, PKN1 (PKNalpha/PRK1/PAK1), PKN2 (PRK2/PAK2/PKNgamma), and PKN3 (PKNbeta). PKN isoforms show different enzymatic properties and tissue distributions and have been implicated in various distinct cellular processes (reviewed in Mukai [2003]). This chapter discusses methods to prepare purified enzymes and to assay substrate phosphorylation activities.

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Year:  2006        PMID： 16472661     DOI： 10.1016/S0076-6879(06)06017-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  7 in total

1.  Identification of an interaction between the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor with protein kinase C-related kinase (PRK) 1: implications for prostate cancer.

Authors:  Elizebeth C Turner; David J Kavanagh; Eamon P Mulvaney; Caitriona McLean; Katarina Wikström; Helen M Reid; B Therese Kinsella
Journal:  J Biol Chem       Date:  2011-02-28       Impact factor: 5.157

2.  Development of an intracellularly acting inhibitory peptide selective for PKN.

Authors:  Kazuhiro Shiga; Kentaro Takayama; Shiroh Futaki; Jessica E Hutti; Lewis C Cantley; Katsuko Ueki; Yoshitaka Ono; Hideyuki Mukai
Journal:  Biochem J       Date:  2009-12-23       Impact factor: 3.857

3.  Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.

Authors:  Matthew D Falk; Wei Liu; Ben Bolaños; Keziban Unsal-Kacmaz; Anke Klippel; Stephan Grant; Alexei Brooun; Sergei Timofeevski
Journal:  Biosci Rep       Date:  2014-04-01       Impact factor: 3.840

Review 4.  PKC and PKN in heart disease.

Authors:  Valeria Marrocco; Julius Bogomolovas; Elisabeth Ehler; Cristobal G Dos Remedios; Jiayu Yu; Chen Gao; Stephan Lange
Journal:  J Mol Cell Cardiol       Date:  2019-02-08       Impact factor: 5.000

5.  1H, 15N and 13C resonance assignments of the HR1c domain of PRK1, a protein kinase C-related kinase.

Authors:  Georgios Sophocleous; George Wood; Darerca Owen; Helen R Mott
Journal:  Biomol NMR Assign       Date:  2020-06-04       Impact factor: 0.746

Review 6.  The structure and function of protein kinase C-related kinases (PRKs).

Authors:  Georgios Sophocleous; Darerca Owen; Helen R Mott
Journal:  Biochem Soc Trans       Date:  2021-02-26       Impact factor: 5.407

7.  Identification of novel off targets of baricitinib and tofacitinib by machine learning with a focus on thrombosis and viral infection.

Authors:  Maria L Faquetti; Francesca Grisoni; Petra Schneider; Gisbert Schneider; Andrea M Burden
Journal:  Sci Rep       Date:  2022-05-12       Impact factor: 4.996
  7 in total

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