| Literature DB >> 16472552 |
Hyo Won Chang1, Sang Yoon Kim, So-Lyoung Yi, Se-Hee Son, Do Young Song, Su Young Moon, Jong Hoon Kim, Eun Kyung Choi, Seung Do Ahn, Seong Soo Shin, Kang Kyoo Lee, Sang-Wook Lee.
Abstract
The Ku protein is essential for the repair of a majority of DNA double-strand breaks in mammalian cells. The purpose of this study was to investigate the relationship between the expression of Ku70/80 and sensitivity to radiation in cancer cell lines of the head and neck. The sensitivity to radiation in various head and neck cancer cell lines (AMC-HN-1 to -9) was analyzed by colony forming assay. Of the nine cell lines examined, the most radiosensitive cell line (AMC-HN-3) and the most radioresistant cell line (AMC-HN-9) were selected for this experiments. The expression of Ku70/80 was examined after irradiation using real time PCR, Western blotting and immunofluorescence in two different cell lines. Cell cycle distribution after irradiation were analysed. A differential radioresponse was demonstrated by expression of Ku70/80 in AMC-HN-3 and AMC-HN-9 cells. While the expression of Ku70 was slightly increased in the radioresistant AMC-HN-9 cell line, the expression of Ku80 was remarkably increased, suggesting a correlation between Ku80 expression and radiation resistance. Overexpression of Ku80 plays an important role in the repair of DNA damage induced by radiation. Ku80 expression may provide an effective predictive assay of radiosensitivity in head and neck cancers.Entities:
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Year: 2006 PMID： 16472552 DOI： 10.1016/j.oraloncology.2005.12.016
Source DB: PubMed Journal: Oral Oncol ISSN： 1368-8375 Impact factor: 5.337