Feng-ying Xiao1, Fu-er Lu, Li-jun Xu. 1. Tongji Hospital, Tongii Medical College, Huazhong University of Science & Technology, Wuhan 430030, China. xiaofengyinglh@sina.com.cn
Abstract
OBJECTIVE: To explore the effects of drug-carried serum of the different parts of Portulace olerace on cytokine TNF-alpha and IL-6 secreted by adipose cell in vitro. METHOD: Models of adipose cell were established by Rodbell method. Using the method of seropharmacology, the drug-carried serum of the different parts of P. olerace were prepared. The cell viability of each group was tested by. Methy thiazolyl tetrazolium (MTT) assay. The levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell were assayed by RIA. RESULT: MTT assay results showed the cell viability of normal serum group was significantly higher than that of high lipid serum( P < 0.05). Compared with the high lipid serum group, the cell viability of the drug-carried serum groups in 40% and 20% concentration were significantly increased( P < 0.05). The high lipid serum had a better effect on increasing the levels of TNF-alpha and IL-6 than the normal serum group (P < 0.01). Expect the drug-carried serum of P. olerace low dose group in 20% concentration, each drug-carried serum group could markedly lower the levels of TNF-alpha (P < 0.05 or P < 0.01). Each drug-carried serum group in 40% concentration and the drug-carried serum P. olerace MS high dose group in 20% concentration could markedly lower the levels of IL-6 (P < 0.05 or P < 0.01). CONCLUSION: The drug-carried serum of P. olerace and its different parts act on adipose cell damaged by the high lipid serum, significantly increas the cell viability in the groups in 40% and 20% concentration, and improve the disorder of lipid in differeut degrel by lowering the levels of TNF-alpha and IL-6 that adipose cell secreted in vitro.
OBJECTIVE: To explore the effects of drug-carried serum of the different parts of Portulace olerace on cytokine TNF-alpha and IL-6 secreted by adipose cell in vitro. METHOD: Models of adipose cell were established by Rodbell method. Using the method of seropharmacology, the drug-carried serum of the different parts of P. olerace were prepared. The cell viability of each group was tested by. Methy thiazolyl tetrazolium (MTT) assay. The levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell were assayed by RIA. RESULT: MTT assay results showed the cell viability of normal serum group was significantly higher than that of high lipid serum( P < 0.05). Compared with the high lipid serum group, the cell viability of the drug-carried serum groups in 40% and 20% concentration were significantly increased( P < 0.05). The high lipid serum had a better effect on increasing the levels of TNF-alpha and IL-6 than the normal serum group (P < 0.01). Expect the drug-carried serum of P. olerace low dose group in 20% concentration, each drug-carried serum group could markedly lower the levels of TNF-alpha (P < 0.05 or P < 0.01). Each drug-carried serum group in 40% concentration and the drug-carried serum P. olerace MS high dose group in 20% concentration could markedly lower the levels of IL-6 (P < 0.05 or P < 0.01). CONCLUSION: The drug-carried serum of P. olerace and its different parts act on adipose cell damaged by the high lipid serum, significantly increas the cell viability in the groups in 40% and 20% concentration, and improve the disorder of lipid in differeut degrel by lowering the levels of TNF-alpha and IL-6 that adipose cell secreted in vitro.