PURPOSE: To verify a more effective dilution method that can be applied to human expanded blastocysts that are vitrified after artificial shrinkage. METHODS: Surplus expanded blastocysts that remained after embryo transfer (ET) in in vitro fertilization (IVF) cycles, were cryopreserved. The blastocysts were vitrified on EM grids following artificial shrinkage. After thawing the blastocysts, cryoprotectants were diluted using either a 6- or 2-step method. We examined the survival rate and clinical outcome of blastocysts of 151 patients in our ET program after thawing. RESULTS: The survival rate of blastocysts that were thawed using a 2-step method (91.6%, 239/261) was comparable with that of the 6-step method (89%, 186/209). The clinical pregnancy rate (45.9%, 39/85) and implantation rate (24.1%, 53/220) were slightly higher in the 2-step method than in the 6-step method (40.9%, 27/66; 19.4%, 33/170). CONCLUSIONS: Our data indicate that the 2-step dilution method could be a simpler and more effective protocol for human expanded blastocysts that are vitrified using EM-grid following artificial shrinkage.
PURPOSE: To verify a more effective dilution method that can be applied to human expanded blastocysts that are vitrified after artificial shrinkage. METHODS: Surplus expanded blastocysts that remained after embryo transfer (ET) in in vitro fertilization (IVF) cycles, were cryopreserved. The blastocysts were vitrified on EM grids following artificial shrinkage. After thawing the blastocysts, cryoprotectants were diluted using either a 6- or 2-step method. We examined the survival rate and clinical outcome of blastocysts of 151 patients in our ET program after thawing. RESULTS: The survival rate of blastocysts that were thawed using a 2-step method (91.6%, 239/261) was comparable with that of the 6-step method (89%, 186/209). The clinical pregnancy rate (45.9%, 39/85) and implantation rate (24.1%, 53/220) were slightly higher in the 2-step method than in the 6-step method (40.9%, 27/66; 19.4%, 33/170). CONCLUSIONS: Our data indicate that the 2-step dilution method could be a simpler and more effective protocol for human expanded blastocysts that are vitrified using EM-grid following artificial shrinkage.
Authors: Pierre Vanderzwalmen; G Bertin; Ch Debauche; V Standaert; E van Roosendaal; M Vandervorst; N Bollen; H Zech; T Mukaida; K Takahashi; R Schoysman Journal: Hum Reprod Date: 2002-03 Impact factor: 6.918
Authors: Michael L Reed; Michelle Lane; David K Gardner; Nancy L Jensen; Jim Thompson Journal: J Assist Reprod Genet Date: 2002-06 Impact factor: 3.412
Authors: P Vanderzwalmen; G Bertin; Ch Debauche; V Standaert; N Bollen; E van Roosendaal; M Vandervorst; R Schoysman; N Zech Journal: Hum Reprod Date: 2003-07 Impact factor: 6.918
Authors: Eun Kyung Ryu; Yong Soo Hur; Ji Young Ann; Ja Young Maeng; Miji Park; Jeong Hyun Park; Jung Yoon; San Hyun Yoon; Chang Young Hur; Won Don Lee; Jin Ho Lim Journal: Clin Exp Reprod Med Date: 2012-12-31