Literature DB >> 1646777

Rapid polymerization of LR-white for immunocytochemistry.

S Hillmer1, S Joachim, D G Robinson.   

Abstract

London Resin (LR) White a hydrophilic embedding medium for immunocytochemistry, can be polymerized in a commercial microwave oven in seven minutes using the chemical accelerator benzoyl peroxide. In order to minimize the effects of heating, the polymerization vessel was maintained in an ice bath. We demonstrate that this procedure does not affect the antigenicity of either barley aleurone nuclease or of the catalytic and regulatory subunits of rat parotid cAMP-dependent protein kinase.

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Year:  1991        PMID: 1646777     DOI: 10.1007/bf00266782

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  12 in total

1.  Biochemical properties and hormonal regulation of barley nuclease.

Authors:  P H Brown; T H Ho
Journal:  Eur J Biochem       Date:  1987-10-15

2.  Barley aleurone protoplasts are structurally and functionally similar to the walled cells of aleurone layers.

Authors:  S Hillmer; D S Bush; D G Robinson; I Zingen-Sell; R L Jones
Journal:  Eur J Cell Biol       Date:  1990-06       Impact factor: 4.492

3.  Immunoelectron microscopic localization of catalytic and regulatory subunits of cAMP-dependent protein kinases in the parotid gland.

Authors:  S Joachim; G Schwoch
Journal:  Eur J Cell Biol       Date:  1988-08       Impact factor: 4.492

4.  Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.

Authors:  J Roth; M Bendayan; L Orci
Journal:  J Histochem Cytochem       Date:  1978-12       Impact factor: 2.479

5.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

6.  Ca-stimulated secretion of alpha-amylase during development in barley aleurone protoplasts.

Authors:  D S Bush; M J Cornejo; C N Huang; R L Jones
Journal:  Plant Physiol       Date:  1986-10       Impact factor: 8.340

7.  Barley aleurone layers secrete a nuclease in response to gibberellic Acid : purification and partial characterization of the associated ribonuclease, deoxyribonuclease, and 3'-nucleotidase activities.

Authors:  P H Brown; T H Ho
Journal:  Plant Physiol       Date:  1986-11       Impact factor: 8.340

8.  Rapid embedding of tissues in Lowicryl K4M for immunoelectron microscopy.

Authors:  L G Altman; B G Schneider; D S Papermaster
Journal:  J Histochem Cytochem       Date:  1984-11       Impact factor: 2.479

9.  Gibberellic-acid-responsive protoplasts from mature aleurone of Himalaya barley.

Authors:  J V Jacobsen; J A Zwar; P M Chandler
Journal:  Planta       Date:  1985-03       Impact factor: 4.116

10.  Potency of microwave irradiation during fixation for electron microscopy.

Authors:  P Wild; M Krähenbühl; E M Schraner
Journal:  Histochemistry       Date:  1989
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  2 in total

1.  Microwave-stimulated glutaraldehyde and osmium tetroxide fixation of plant tissue: ultrastructural preservation in seconds.

Authors:  H G Heumann
Journal:  Histochemistry       Date:  1992-05

2.  Rapid diagnostic thin section electron microscopy of bacterial endospores.

Authors:  Michael Laue; Bärbel Niederwöhrmeier; Norbert Bannert
Journal:  J Microbiol Methods       Date:  2007-03-30       Impact factor: 2.363

  2 in total

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