Literature DB >> 16467482

Metallothionein gene from Tetrahymena thermophila with a copper-inducible-repressible promoter.

Francesco Boldrin1, Gianfranco Santovito, Jacek Gaertig, Dorota Wloga, Donna Cassidy-Hanley, Theodore G Clark, Ester Piccinni.   

Abstract

We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.

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Year:  2006        PMID： 16467482      PMCID： PMC1405887          DOI： 10.1128/EC.5.2.422-425.2006

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  12 in total

1.  Ciliates display promise for foreign gene expression.

Authors:  C F Brunk
Journal:  Nat Biotechnol       Date:  1999-05       Impact factor: 54.908

2.  Cadmium metallothionein gene of Tetrahymena pyriformis.

Authors:  E Piccinni; D Bertaggia; G Santovito; C Miceli; A Kraev
Journal:  Gene       Date:  1999-06-24       Impact factor: 3.688

3.  Identification, cloning and characterisation of a novel copper-metallothionein in tetrahymena pigmentosa. Sequencing of cDNA and expression.

Authors:  G Santovito; P Irato; S Palermo; F Boldrin; R Sack; P Hunziker; E L Piccinni
Journal:  Protist       Date:  2001-09

4.  Metal interaction and regulation of Tetrahymena pigmentosa metallothionein genes.

Authors:  Francesco Boldrin; Gianfranco Santovito; Paola Irato; Ester Piccinni
Journal:  Protist       Date:  2002-09

5.  Surface display of a parasite antigen in the ciliate Tetrahymena thermophila.

Authors:  J Gaertig; Y Gao; T Tishgarten; T G Clark; H W Dickerson
Journal:  Nat Biotechnol       Date:  1999-05       Impact factor: 54.908

Review 6.  Induction, regulation, degradation, and biological significance of mammalian metallothioneins.

Authors:  A T Miles; G M Hawksworth; J H Beattie; V Rodilla
Journal:  Crit Rev Biochem Mol Biol       Date:  2000       Impact factor: 8.250

7.  A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.

Authors:  Yuhua Shang; Xiaoyuan Song; Josephine Bowen; Robert Corstanje; Yan Gao; Jacek Gaertig; Martin A Gorovsky
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-12       Impact factor: 11.205

8.  Cloning and sequencing of four new metallothionein genes from Tetrahymena thermophila and T. pigmentosa: evolutionary relationships in Tetrahymena MT family.

Authors:  Francesco Boldrin; Gianfranco Santovito; Enrico Negrisolo; Ester Piccinni
Journal:  Protist       Date:  2003-10

9.  Cadmium effects on Ichthyophthirius: evidence for metal-sequestration in fish tissues following administration of recombinant vaccines.

Authors:  Y Bisharyan; Q Chen; M M Hossain; A Papoyan; T G Clark
Journal:  Parasitology       Date:  2003       Impact factor: 3.234

10.  Purification and primary structure of metallothioneins induced by cadmium in the protists Tetrahymena pigmentosa and Tetrahymena pyriformis.

Authors:  E Piccinni; W Staudenmann; V Albergoni; R De Gabrieli; P James
Journal:  Eur J Biochem       Date:  1994-12-15
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  11 in total

1.  The Tetrahymena thermophila phagosome proteome.

Authors:  Mary Ellen Jacobs; Leroi V DeSouza; Haresha Samaranayake; Ronald E Pearlman; K W Michael Siu; Lawrence A Klobutcher
Journal:  Eukaryot Cell       Date:  2006-09-29

2.  Characterization of the peroxiredoxin 1 subfamily from Tetrahymena thermophila.

Authors:  Sarmad Al-Asadi; Arif Malik; Rigers Bakiu; Gianfranco Santovito; Ian Menz; Kathryn Schuller
Journal:  Cell Mol Life Sci       Date:  2019-05-25       Impact factor: 9.261

3.  Molecular Determinants of Tubulin's C-Terminal Tail Conformational Ensemble.

Authors:  Kathryn P Wall; Maria Pagratis; Geoffrey Armstrong; Jeremy L Balsbaugh; Eric Verbeke; Chad G Pearson; Loren E Hough
Journal:  ACS Chem Biol       Date:  2016-09-28       Impact factor: 5.100

4.  Transcriptional profiling of antioxidant defense system and heat shock protein (Hsp) families in the cadmium- and copper-exposed marine ciliate Euplotes crassu.

Authors:  Bo-Mi Kim; Jae-Sung Rhee; Ik-Young Choi; Young-Mi Lee
Journal:  Genes Genomics       Date:  2017-10-16       Impact factor: 1.839

5.  Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena.

Authors:  Kristin M Slade; Sydney Freggiaro; Kyle A Cottrell; Joshua J Smith; Emily A Wiley
Journal:  BMC Cell Biol       Date:  2011-09-21       Impact factor: 4.241

6.  Tetrahymena metallothioneins fall into two discrete subfamilies.

Authors:  Silvia Díaz; Francisco Amaro; Daniel Rico; Virginia Campos; Laura Benítez; Ana Martín-González; Eileen P Hamilton; Eduardo Orias; Juan C Gutiérrez
Journal:  PLoS One       Date:  2007-03-14       Impact factor: 3.240

7.  Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila.

Authors:  Gürkan Yilmaz; Muhittin Arslanyolu
Journal:  BMC Biotechnol       Date:  2015-03-25       Impact factor: 2.563

8.  Hints for metal-preference protein sequence determinants: different metal binding features of the five tetrahymena thermophila metallothioneins.

Authors:  Anna Espart; Maribel Marín; Selene Gil-Moreno; Òscar Palacios; Francisco Amaro; Ana Martín-González; Juan C Gutiérrez; Mercè Capdevila; Sílvia Atrian
Journal:  Int J Biol Sci       Date:  2015-03-18       Impact factor: 6.580

9.  Extreme metal adapted, knockout and knockdown strains reveal a coordinated gene expression among different Tetrahymena thermophila metallothionein isoforms.

Authors:  Patricia de Francisco; Ana Martín-González; Aaron P Turkewitz; Juan Carlos Gutiérrez
Journal:  PLoS One       Date:  2017-12-05       Impact factor: 3.240

10.  Recombinational micro-evolution of functionally different metallothionein promoter alleles from Orchesella cincta.

Authors:  Thierry K S Janssens; Janine Mariën; Peter Cenijn; J Legler; Nico M van Straalen; Dick Roelofs
Journal:  BMC Evol Biol       Date:  2007-06-11       Impact factor: 3.260
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