OBJECTIVE: To determine the possible role of the plasminogen activator (PA)/plasmin system in gouty arthritis. METHODS: We compared the antigenic values of urokinase-type PA (uPA), soluble uPA receptor (suPAR), and PA inhibitor type-1 (PAI-1) and the levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 in 63 synovial fluid (SF) samples from knee joints of 38 patients with gouty arthritis and 20 SF samples from knee joints of 20 patients with osteoarthritis (OA). RESULTS: The increases of uPA, suPAR, and PAI-1 antigenic values in SF were associated with increased levels of latent MMP-9 (pro-MMP-9) (p < 0.001, p < 0.001, p < 0.001, respectively) in gouty arthritis, whereas a correlation was not observed between uPA, suPAR, and PAI-1 antigenic values and MMP-2 levels. Increased uPA, suPAR, and PAI-1 antigenic values in gouty arthritis SF were also correlated to each other (p < 0.001, p < 0.001, p < 0.001). In gouty arthritis SF, no significant relations were observed between uPA and suPAR antigenic values and leukocyte, neutrophil, or monocyte counts, while increased values of PAI-1 corresponded closely with increased leukocyte and neutrophil counts (p = 0.005, p = 0.004). Significantly higher values of the uPA, suPAR, and PAI-1 (p < 0.001, p < 0.001, p = 0.012) appeared in SF of gouty arthritis than in SF from patients with OA. CONCLUSION: These results show a correlation of the PA/plasmin system and MMP-9 in SF from patients with gouty arthritis, resulting in increased PA catalytic activity and contributing to inflammation in gouty arthritis.
OBJECTIVE: To determine the possible role of the plasminogen activator (PA)/plasmin system in gouty arthritis. METHODS: We compared the antigenic values of urokinase-type PA (uPA), soluble uPA receptor (suPAR), and PA inhibitor type-1 (PAI-1) and the levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 in 63 synovial fluid (SF) samples from knee joints of 38 patients with gouty arthritis and 20 SF samples from knee joints of 20 patients with osteoarthritis (OA). RESULTS: The increases of uPA, suPAR, and PAI-1 antigenic values in SF were associated with increased levels of latent MMP-9 (pro-MMP-9) (p < 0.001, p < 0.001, p < 0.001, respectively) in gouty arthritis, whereas a correlation was not observed between uPA, suPAR, and PAI-1 antigenic values and MMP-2 levels. Increased uPA, suPAR, and PAI-1 antigenic values in gouty arthritis SF were also correlated to each other (p < 0.001, p < 0.001, p < 0.001). In gouty arthritis SF, no significant relations were observed between uPA and suPAR antigenic values and leukocyte, neutrophil, or monocyte counts, while increased values of PAI-1 corresponded closely with increased leukocyte and neutrophil counts (p = 0.005, p = 0.004). Significantly higher values of the uPA, suPAR, and PAI-1 (p < 0.001, p < 0.001, p = 0.012) appeared in SF of gouty arthritis than in SF from patients with OA. CONCLUSION: These results show a correlation of the PA/plasmin system and MMP-9 in SF from patients with gouty arthritis, resulting in increased PA catalytic activity and contributing to inflammation in gouty arthritis.
Authors: Ping-Kun Tsai; Shih-Ming Tsao; Wei-En Yang; Chao-Bin Yeh; Hsiang-Ling Wang; Shun-Fa Yang Journal: Int J Environ Res Public Health Date: 2019-03-21 Impact factor: 3.390
Authors: Michael Boehm; Eva Nora Bukosza; Nicole Huttary; Rebecca Herzog; Christoph Aufricht; Klaus Kratochwill; Christoph A Gebeshuber Journal: PLoS One Date: 2019-03-28 Impact factor: 3.240
Authors: Yubo Sun; Andrea Roberts; David R Mauerhan; Andrew R Sun; H James Norton; Edward N Hanley Journal: Biomed Res Int Date: 2013-07-11 Impact factor: 3.411