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Literature DB >> 16465

A new semi-defined medium for Trypanosoma brucei sspp.

Abstract

A new, easy to prepare semi-defined medium for the cultivation of trypanosomes of the T. brucei complex is introduced. Containing the two commercially available media MEM (Minimum Essential Medium) and Medium 199 TC45 as well as 10% inactivated foetal calf serum (fcs), the medium supports optimum growth and direct adaptation of bloodstream forms. Growth characteristics, glucose uptake, amino acid utilization and the ultrastructure of trypanosomes grown in this medium are described briefly.

Entities: Chemical Species

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Year: 1977 PMID： 16465

Source DB: PubMed Journal: Acta Trop ISSN： 0001-706X Impact factor: 3.112

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Cited

14 in total

1. The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function.

Authors: Jan-Peter Daniels; Keith Gull; Bill Wickstead
Journal: Eukaryot Cell Date: 2012-03-02

2. Diversification of function by different isoforms of conventionally shared RNA polymerase subunits.

Authors: Sara Devaux; Steven Kelly; Laurence Lecordier; Bill Wickstead; David Perez-Morga; Etienne Pays; Luc Vanhamme; Keith Gull
Journal: Mol Biol Cell Date: 2007-01-31 Impact factor: 4.138

3. Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei.

Authors: Ali Kassem; Etienne Pays; Luc Vanhamme
Journal: Proc Natl Acad Sci U S A Date: 2014-06-02 Impact factor: 11.205

4. Cultivation of infective forms of Trypanosoma (T.) brucei evansi in a continuous culture system.

Authors: E Zweygarth; J S Ahmed; G Rehbein
Journal: Z Parasitenkd Date: 1983

5. Cultivation of procyclic trypomastigotes of Trypanosoma congolense in a semi-defined medium with direct adaptation from bloodstream forms.

Authors: R Brun
Journal: Z Parasitenkd Date: 1982

6. Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections.

Authors: Galina I Lepesheva; Hee-Won Park; Tatiana Y Hargrove; Benoit Vanhollebeke; Zdzislaw Wawrzak; Joel M Harp; Munirathinam Sundaramoorthy; W David Nes; Etienne Pays; Minu Chaudhuri; Fernando Villalta; Michael R Waterman
Journal: J Biol Chem Date: 2009-11-18 Impact factor: 5.157

A new semi-defined medium for Trypanosoma brucei sspp.

1. The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function.

2. Diversification of function by different isoforms of conventionally shared RNA polymerase subunits.

3. Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei.

4. Cultivation of infective forms of Trypanosoma (T.) brucei evansi in a continuous culture system.

5. Cultivation of procyclic trypomastigotes of Trypanosoma congolense in a semi-defined medium with direct adaptation from bloodstream forms.

6. Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections.

7. Use of antigens of cultured Trypanosoma brucei in tests for bovine trypanosomiasis.

8. Properties of a blood-meal-induced midgut lectin from the tsetse fly Glossina morsitans.

9. Centromere-associated repeat arrays on Trypanosoma brucei chromosomes are much more extensive than predicted.

10. A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod.