Literature DB >> 16461730

Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor.

Tim J Strovas1, Joe M Dragavon, Tyler J Hankins, James B Callis, Lloyd W Burgess, Mary E Lidstrom.   

Abstract

Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes.

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Year:  2006        PMID： 16461730      PMCID： PMC1392911          DOI： 10.1128/AEM.72.2.1692-1695.2006

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  20 in total

Review 1.  Energy model and metabolic flux analysis for autotrophic nitrifiers.

Authors:  L Poughon; C G Dussap; J B Gros
Journal:  Biotechnol Bioeng       Date:  2001-02-20       Impact factor: 4.530

Review 2.  The Na+-translocating NADH:quinone oxidoreductase (NDH I) from Klebsiella pneumoniae and Escherichia coli: implications for the mechanism of redox-driven cation translocation by complex I.

Authors:  J Steuber
Journal:  J Bioenerg Biomembr       Date:  2001-06       Impact factor: 2.945

3.  Overexpression of a heterologous protein, haloalkane dehalogenase, in a poly-beta-hydroxybutyrate-deficient strain of the facultative methylotroph Methylobacterium extorquens AM1.

Authors:  Kelly A FitzGerald; Mary E Lidstrom
Journal:  Biotechnol Bioeng       Date:  2003-02-05       Impact factor: 4.530

Review 4.  Biological methane oxidation: regulation, biochemistry, and active site structure of particulate methane monooxygenase.

Authors:  Raquel L Lieberman; Amy C Rosenzweig
Journal:  Crit Rev Biochem Mol Biol       Date:  2004 May-Jun       Impact factor: 8.250

5.  Stoichiometric model for evaluating the metabolic capabilities of the facultative methylotroph Methylobacterium extorquens AM1, with application to reconstruction of C(3) and C(4) metabolism.

Authors:  Stephen J Van Dien; Mary E Lidstrom
Journal:  Biotechnol Bioeng       Date:  2002-05-05       Impact factor: 4.530

6.  Determination of metabolic activity of streptomycetes in soil microcosms.

Authors:  E A Katsifas; T G Koraki; A D Karagouni
Journal:  J Appl Microbiol       Date:  2000-07       Impact factor: 3.772

7.  Oxygen solubilities of media used in electrochemical respiration measurements.

Authors:  Hans N Rasmussen; Ulla F Rasmussen
Journal:  Anal Biochem       Date:  2003-08-01       Impact factor: 3.365

8.  Continuous oxygen monitoring of mammalian cell growth on space shuttle mission STS-93 with a novel radioluminescent oxygen sensor.

Authors:  Julie S Reece; Michael J Miller; Mark A Arnold; Cris Waterhouse; Ted Delaplaine; Laura Cohn; Tom Cannon
Journal:  Appl Biochem Biotechnol       Date:  2003-01       Impact factor: 2.926

9.  Reconstruction of C(3) and C(4) metabolism in Methylobacterium extorquens AM1 using transposon mutagenesis.

Authors:  Stephen J Van Dien; Yoko Okubo; Melinda T Hough; Natalia Korotkova; Tricia Taitano; Mary E Lidstrom
Journal:  Microbiology       Date:  2003-03       Impact factor: 2.777

10.  Poly-beta-hydroxybutyrate biosynthesis in the facultative methylotroph methylobacterium extorquens AM1: identification and mutation of gap11, gap20, and phaR.

Authors:  Natalia Korotkova; Ludmila Chistoserdova; Mary E Lidstrom
Journal:  J Bacteriol       Date:  2002-11       Impact factor: 3.490
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  2 in total

1.  Direct measurement of oxygen consumption rates from attached and unattached cells in a reversibly sealed, diffusionally isolated sample chamber.

Authors:  Timothy J Strovas; Sarah C McQuaide; Judy B Anderson; Vivek Nandakumar; Marina G Kalyuzhnaya; Lloyd W Burgess; Mark R Holl; Deirdre R Meldrum; Mary E Lidstrom
Journal:  Adv Biosci Biotechnol       Date:  2010-12-01

2.  A cellular isolation system for real-time single-cell oxygen consumption monitoring.

Authors:  Joe Dragavon; Tim Molter; Cody Young; Tim Strovas; Sarah McQuaide; Mark Holl; Meng Zhang; Brad Cookson; Alex Jen; Mary Lidstrom; Deirdre Meldrum; Lloyd Burgess
Journal:  J R Soc Interface       Date:  2008-10-06       Impact factor: 4.118
  2 in total

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