Literature DB >> 16461019

Real-time assay of immobilized tannase with a stopped-flow conductometric device.

Fu-Shiang Chang1, Po-Chung Chen, Richie L C Chen, Fu-Ming Lu, Tzong-Jih Cheng.   

Abstract

A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant=103.2 cm(-1)) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.

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Year:  2006        PMID: 16461019     DOI: 10.1016/j.bioelechem.2005.12.004

Source DB:  PubMed          Journal:  Bioelectrochemistry        ISSN: 1567-5394            Impact factor:   5.373


  2 in total

1.  Catalytical Properties of Free and Immobilized Aspergillus niger Tannase.

Authors:  Abril Flores-Maltos; Luis V Rodríguez-Durán; Jacqueline Renovato; Juan C Contreras; Raúl Rodríguez; Cristóbal N Aguilar
Journal:  Enzyme Res       Date:  2011-09-12

2.  Flow-based systems for rapid and high-precision enzyme kinetics studies.

Authors:  Supaporn Kradtap Hartwell; Kate Grudpan
Journal:  J Anal Methods Chem       Date:  2012-04-22       Impact factor: 2.193

  2 in total

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