| Literature DB >> 16455275 |
Marcella Reale1, Maria Anna De Lutiis, Antonia Patruno, Lorenza Speranza, Mario Felaco, Alfredo Grilli, Maria Antonietta Macrì, Silvia Comani, Pio Conti, Silvano Di Luzio.
Abstract
The purpose of this study was to investigate whether overnight exposure to 1 mT-50 Hz extremely low-frequency sinusoidal electromagnetic field (EMF) affects the expression and production of inducible nitric oxide synthase (iNOS) and monocyte chemotactic protein-1 (MCP-1) in human monocytes. RT-PCR and Western blot analysis demonstrate that EMF exposure affects the expression of iNOS and MCP-1 in cultured human mononuclear cells at the mRNA level and protein synthesis. Interestingly, the effects of EMF exposure clearly differed with respect to the potentiation and inhibition of iNOS and MCP-1 expression. Whereas iNOS was down-regulated both at the mRNA level and at the protein level, MCP-1 was up-regulated. These results provide helpful information regarding the EMF-mediated modulation of the inflammatory response in vivo. However, additional studies are necessary to demonstrate that EMF acts as a nonpharmacological inhibitor of NO and inducer of MCP-1 in some diseases where the balance of MCP-1 and NO may be important.Entities:
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Year: 2006 PMID： 16455275 DOI： 10.1016/j.niox.2005.11.010
Source DB: PubMed Journal: Nitric Oxide ISSN： 1089-8603 Impact factor: 4.427