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Literature DB >> 16453461

Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene.

J A Downie¹, Q S Ma, C D Knight, G Hombrecher, A W Johnston.

Abstract

The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones. These clones represent 60 kb of pRL1JI DNA on which a series of Tn5-induced fix and nod alleles have been identified, with the two most distant alleles being separated by 45 kb of DNA. The mutant alleles fell into three groups, two clusters of fix alleles separated by one cluster of nod alleles. Within one group of fix alleles, DNA homologous to the nifA gene of Klebsiella pneumoniae has been identified, whereas the pRL1JI DNA homologous to the K. pneumoniae nitrogenase genes is present within the other group of fix alleles.

Entities: Chemical

Year: 1983 PMID： 16453461 PMCID： PMC555213 DOI： 10.1002/j.1460-2075.1983.tb01526.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

19 in total

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors: M Grunstein; D S Hogness
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

2. In vitro packaging of lambda and cosmid DNA.

Authors: B Hohn
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

5. R factor transfer in Rhizobium leguminosarum.

Authors: J E Beringer
Journal: J Gen Microbiol Date: 1974-09

6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

7. Role of the nifA gene product in the regulation of nif expression in Klebsiella pneumoniae.

Authors: V Buchanan-Wollaston; M C Cannon; J L Beynon; F C Cannon
Journal: Nature Date: 1981-12-24 Impact factor: 49.962

8. Recovery of DNA from gels.

Authors: H O Smith
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

9. Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster.

Authors: G Espin; A Alvarez-Morales; F Cannon; R Dixon; M Merrick
Journal: Mol Gen Genet Date: 1982

10. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.

Authors: A M Friedman; S R Long; S E Brown; W J Buikema; F M Ausubel
Journal: Gene Date: 1982-06 Impact factor: 3.688

61 in total

1. Either of two nod gene loci can complement the nodulation defect of a nod deletion mutant of Rhizobium leguminosarum bv viciae.

Authors: J A Downie; B P Surin
Journal: Mol Gen Genet Date: 1990-06

2. Sym2 of Pea Is Involved in a Nodulation Factor-Perception Mechanism That Controls the Infection Process in the Epidermis.

Authors: R. Geurts; R. Heidstra; A. E. Hadri; J. A. Downie; H. Franssen; A. Van Kammen; T. Bisseling
Journal: Plant Physiol Date: 1997-10 Impact factor: 8.340

3. Molecular characterization and regulation of the rhizosphere-expressed genes rhiABCR that can influence nodulation by Rhizobium leguminosarum biovar viciae.

Authors: M T Cubo; A Economou; G Murphy; A W Johnston; J A Downie
Journal: J Bacteriol Date: 1992-06 Impact factor: 3.490

8. Rhizobium meliloti nodulation genes allow Agrobacterium tumefaciens and Escherichia coli to form pseudonodules on alfalfa.

Authors: A M Hirsch; K J Wilson; J D Jones; M Bang; V V Walker; F M Ausubel
Journal: J Bacteriol Date: 1984-06 Impact factor: 3.490

9. Identification of a rhizosphere protein encoded by the symbiotic plasmid of Rhizobium leguminosarum.

Authors: N J Dibb; J A Downie; N J Brewin
Journal: J Bacteriol Date: 1984-05 Impact factor: 3.490

10. Isolation of a DNA polymerase I (polA) mutant of Rhizobium leguminosarum that has significantly reduced levels of an IncQ-group plasmid.

Authors: S F Crank; J A Downie
Journal: Mol Gen Genet Date: 1994-04