OBJECTIVE: This study was undertaken to evaluate whether the effects of hyperbaric oxygen (HBO) therapy could alter the critical size for spontaneous healing of a bone defect in the rabbit calvarial model. STUDY DESIGN: An animal trial of 12 weeks duration was conducted using 20 New Zealand white rabbits, which were randomly divided into 2 groups of 10 animals each. Calvarial defects were created in the parietal bones of each animal bilaterally. Defects were critical-sized, 15 mm on one side and supra-critical-sized, 18 mm on the contralateral side. Group 1 received a 90-min HBO treatment sessions at 2.4 absolute atmospheric pressure (ATA) per day for 20 consecutive days. Group 2 served as a control without any HBO treatment sessions. Five animals in each group were sacrificed at 6 and 12 weeks. Data analysis included qualitative assessment of the calvarial specimens, post-sacrifice radiographs, as well as histomorphometric analysis to compute the amount of regenerated bone within the defects. ANOVA and paired sample t test were used for statistical analysis. RESULTS: Both radiographic analysis and histomorphometric analysis demonstrated that HBO-treated animals had significantly more new bone within their defects compared with the control group (P < .001). There was no statistically significant difference between the percentage of new bone forming in the 15-mm and 18-mm HBO-treated defects. There was no difference between the 6-week and the 12-week HBO-treated groups. HBO is effective in enhancing the bony healing of full thickness critical sized as well as supra-critical-sized defects in the rabbit calvarial model. CONCLUSION: Bone regeneration was significantly greater in the HBO-treated animals regardless of the defect size. HBO may have increased the diameter of the rabbit critical-sized calvarial defect to more than 18 mm.
OBJECTIVE: This study was undertaken to evaluate whether the effects of hyperbaric oxygen (HBO) therapy could alter the critical size for spontaneous healing of a bone defect in the rabbit calvarial model. STUDY DESIGN: An animal trial of 12 weeks duration was conducted using 20 New Zealand white rabbits, which were randomly divided into 2 groups of 10 animals each. Calvarial defects were created in the parietal bones of each animal bilaterally. Defects were critical-sized, 15 mm on one side and supra-critical-sized, 18 mm on the contralateral side. Group 1 received a 90-min HBO treatment sessions at 2.4 absolute atmospheric pressure (ATA) per day for 20 consecutive days. Group 2 served as a control without any HBO treatment sessions. Five animals in each group were sacrificed at 6 and 12 weeks. Data analysis included qualitative assessment of the calvarial specimens, post-sacrifice radiographs, as well as histomorphometric analysis to compute the amount of regenerated bone within the defects. ANOVA and paired sample t test were used for statistical analysis. RESULTS: Both radiographic analysis and histomorphometric analysis demonstrated that HBO-treated animals had significantly more new bone within their defects compared with the control group (P < .001). There was no statistically significant difference between the percentage of new bone forming in the 15-mm and 18-mm HBO-treated defects. There was no difference between the 6-week and the 12-week HBO-treated groups. HBO is effective in enhancing the bony healing of full thickness critical sized as well as supra-critical-sized defects in the rabbit calvarial model. CONCLUSION: Bone regeneration was significantly greater in the HBO-treated animals regardless of the defect size. HBO may have increased the diameter of the rabbit critical-sized calvarial defect to more than 18 mm.
Authors: Olli-Pekka Lappalainen; Riikka Korpi; Marianne Haapea; Jarkko Korpi; Leena P Ylikontiola; Soili Kallio-Pulkkinen; Willy S Serlo; Petri Lehenkari; George K Sándor Journal: Childs Nerv Syst Date: 2014-11-13 Impact factor: 1.475
Authors: Olli-Pekka Lappalainen; Sakari Karhula; Marianne Haapea; Laura Kyllönen; Suvi Haimi; Susanna Miettinen; Simo Saarakkala; Jarkko Korpi; Leena P Ylikontiola; Willy S Serlo; George K Sándor Journal: Childs Nerv Syst Date: 2016-01-19 Impact factor: 1.475
Authors: J P Grassmann; J Schneppendahl; M Sager; A R Hakimi; M Herten; T T Loegters; M Wild; M Hakimi; J Windolf; P Jungbluth Journal: J Mater Sci Mater Med Date: 2015-01-11 Impact factor: 3.896
Authors: Hanna Pihlman; Pauli Keränen; Kaarlo Paakinaho; Jere Linden; Markus Hannula; Iida-Kaisa Manninen; Jari Hyttinen; Mikko Manninen; Outi Laitinen-Vapaavuori Journal: J Mater Sci Mater Med Date: 2018-10-08 Impact factor: 3.896