Literature DB >> 1644529

Detection of Opisthorchis viverrini antigens in stools using specific monoclonal antibody.

W Chaicumpa1, L Ybanez, V Kitikoon, S Pungpak, Y Ruangkunaporn, M Chongsa-nguan, S Sornmani.   

Abstract

Detection of Opisthorchis viverrini antigens in stools using specific monoclonal antibody. International Journal for Parasitology 22: 527-531. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detecting Opisthorchis viverrini antigen in faecal extracts of four groups of individuals. These were 24 patients with O. viverrini infection only (group 1), 31 patients with O. viverrini and other parasitic infections (group 2), 141 patients with other parasitic infections (group 3) and 21 normal, parasite-free individuals (group 4). The first antibody used in the ELISA was polyclonal immunoglobulin G prepared from the serum of a rabbit previously immunized with crude extract of O. viverrini. The second antibody was monoclonal antibody specific to an antigen located in the worm tegument and muscular tissue. Sensitivity of the assay was 31% while specificity was 100%. Considerations for improving the sensitivity are discussed.

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Year:  1992        PMID： 1644529     DOI： 10.1016/0020-7519(92)90155-e

Source DB:  PubMed          Journal:  Int J Parasitol        ISSN： 0020-7519            Impact factor:   3.981


  8 in total

Review 1.  Opisthorchis viverrini: the carcinogenic human liver fluke.

Authors:  Natthawut Kaewpitoon; Soraya-J Kaewpitoon; Prasit Pengsaa; Banchob Sripa
Journal:  World J Gastroenterol       Date:  2008-02-07       Impact factor: 5.742

2.  Diagnosis of early infection and post chemotherapeutic treatment by copro-DNA detection in experimental opisthorchiasis.

Authors:  Kunyarat Duenngai; Thidarut Boonmars; Jiraporn Sithithaworn; Paiboon Sithithaworn
Journal:  Parasitol Res       Date:  2012-09-30       Impact factor: 2.289

3.  Effects of day-to-day variation of Opisthorchis viverrini antigen in urine on the accuracy of diagnosing opisthorchiasis in Northeast Thailand.

Authors:  Chanika Worasith; Phattharaphon Wongphutorn; Chutima Homwong; Kulthida Y Kopolrat; Anchalee Techasen; Raynoo Thanan; Chatanun Eamudomkarn; Chompunoot Wangboon; Narong Khuntikeo; Watcharin Loilome; Jiraporn Sithithaworn; Thomas Crellen; Paiboon Sithithaworn
Journal:  PLoS One       Date:  2022-07-19       Impact factor: 3.752

4.  Application of Urine and Copro Antigen Assays after Primary Infection and Drug Treatment in an Experimental Opisthorchiasis Animal Model.

Authors:  Chanika Worasith; Kulthida Y Kopolrat; Opal Pitaksakulrat; Chutima Homwong; Yingpinyapat Kittirat; Phattharaphon Wongphutorn; Jiraporn Sithithaworn; Paiboon Sithithaworn
Journal:  Am J Trop Med Hyg       Date:  2022-03-28       Impact factor: 3.707

5.  Selective Agonists and Antagonists of α9 Versus α7 Nicotinic Acetylcholine Receptors.

Authors:  Roger L Papke; Hina Andleeb; Clare Stokes; Marta Quadri; Nicole A Horenstein
Journal:  ACS Chem Neurosci       Date:  2022-02-15       Impact factor: 5.780

6.  Advances in the Diagnosis of Human Opisthorchiasis: Development of Opisthorchis viverrini Antigen Detection in Urine.

Authors:  Chanika Worasith; Christine Kamamia; Anna Yakovleva; Kunyarat Duenngai; Chompunoot Wangboon; Jiraporn Sithithaworn; Nattaya Watwiengkam; Nisana Namwat; Anchalee Techasen; Watcharin Loilome; Puangrat Yongvanit; Alex Loukas; Paiboon Sithithaworn; Jeffrey M Bethony
Journal:  PLoS Negl Trop Dis       Date:  2015-10-20

7.  Comparing the performance of urine and copro-antigen detection in evaluating Opisthorchis viverrini infection in communities with different transmission levels in Northeast Thailand.

Authors:  Chanika Worasith; Chompunoot Wangboon; Kunyarat Duenngai; Nadda Kiatsopit; Kulthida Kopolrat; Anchalee Techasen; Jiraporn Sithithaworn; Narong Khuntikeo; Watcharin Loilome; Nisana Namwat; Puangrat Yongvanit; Elizabeth J Carlton; Paiboon Sithithaworn
Journal:  PLoS Negl Trop Dis       Date:  2019-02-08

Review 8.  Current Perspectives on Opisthorchiasis Control and Cholangiocarcinoma Detection in Southeast Asia.

Authors:  Narong Khuntikeo; Attapol Titapun; Watcharin Loilome; Puangrat Yongvanit; Bandit Thinkhamrop; Nittaya Chamadol; Thidarat Boonmars; Teerachai Nethanomsak; Ross H Andrews; Trevor N Petney; Paiboon Sithithaworn
Journal:  Front Med (Lausanne)       Date:  2018-04-30
  8 in total

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