UNLABELLED: The effect of 30 minutes of passive stretch of the rat soleus muscle on the myogenic differentiation, myostatin, and atrogin-1 gene expressions. OBJECTIVE: To evaluate the effect of passive stretch, applied for 30 minutes to the rat soleus muscle, on the myogenic differentiation (myoD), myostatin, and atrogin-1 gene expressions. DESIGN: Case-controlled study. SETTING: University laboratory. ANIMALS: Fifty 12-week-old male Wistar rats. INTERVENTIONS: Six groups of animals were given a single stretch bout and were evaluated immediately and 8, 24, 48, 72, and 168 hours later. Another 3 groups were evaluated immediately after 2, 3, and 7 stretches. An intact control group was also analyzed. MAIN OUTCOME MEASURES: The messenger ribonucleic acid (mRNA) levels of myoD, myostatin, and atrogin-1 were assessed by real-time polymerase chain reaction. RESULTS: Twenty-four hours after a single session of stretch only, the myoD mRNA levels had increased compared with the control group, whereas an increase in the atrogin-1 expression was observed after 2, 3, and 7 stretches. CONCLUSIONS: A single session of passive stretch increased the myoD gene expression, a factor related to muscle growth. Interestingly, daily stretches increased the atrogin-1 gene expression, a gene primarily associated with muscle atrophy. The results indicated that gene expression was responsive to the number of stretch sessions.
UNLABELLED: The effect of 30 minutes of passive stretch of the rat soleus muscle on the myogenic differentiation, myostatin, and atrogin-1 gene expressions. OBJECTIVE: To evaluate the effect of passive stretch, applied for 30 minutes to the rat soleus muscle, on the myogenic differentiation (myoD), myostatin, and atrogin-1 gene expressions. DESIGN: Case-controlled study. SETTING: University laboratory. ANIMALS: Fifty 12-week-old male Wistar rats. INTERVENTIONS: Six groups of animals were given a single stretch bout and were evaluated immediately and 8, 24, 48, 72, and 168 hours later. Another 3 groups were evaluated immediately after 2, 3, and 7 stretches. An intact control group was also analyzed. MAIN OUTCOME MEASURES: The messenger ribonucleic acid (mRNA) levels of myoD, myostatin, and atrogin-1 were assessed by real-time polymerase chain reaction. RESULTS: Twenty-four hours after a single session of stretch only, the myoD mRNA levels had increased compared with the control group, whereas an increase in the atrogin-1 expression was observed after 2, 3, and 7 stretches. CONCLUSIONS: A single session of passive stretch increased the myoD gene expression, a factor related to muscle growth. Interestingly, daily stretches increased the atrogin-1 gene expression, a gene primarily associated with muscle atrophy. The results indicated that gene expression was responsive to the number of stretch sessions.
Authors: Lívia Assis; Ana Iochabel Soares Moretti; Thalita Balsamo Abrahão; Heraldo Possolo de Souza; Michael R Hamblin; Nivaldo Antonio Parizotto Journal: Lasers Med Sci Date: 2012-08-17 Impact factor: 3.161
Authors: Thiago L Russo; Sabrina M Peviani; João L Q Durigan; Davilene Gigo-Benato; Gabriel B Delfino; Tania F Salvini Journal: J Muscle Res Cell Motil Date: 2010-02-27 Impact factor: 2.698
Authors: Sabrina M Peviani; Vinicius Guzzoni; Clara M Pinheiro-Dardis; Yara P da Silva; Alisson C R Fioravante; Adriana H Sagawa; Gabriel B Delfino; João L Q Durigan; Tania F Salvini Journal: Sci Rep Date: 2018-06-13 Impact factor: 4.379