Literature DB >> 16442733

Obtaining TEM images with a uniform deviation parameter.

Alwyn Eades1.   

Abstract

In transmission electron microscopes made during the last quarter of a century, it has been impossible to take images in which the diffraction contrast conditions are uniform across the field of view. This is inconvenient when, for example, imaging dislocations at a relatively low magnification. The problem arises because modern microscopes use immersion lenses in which the sample sits in a high magnetic field. The resulting helical trajectories of the electrons at the sample plane mean that it is not possible to make a beam that is parallel at the sample. Results of a method to overcome this problem are presented. It is shown that a simple modification to the microscope (which, on a computer-controlled microscope, could be implemented in software) can be used to produce images in which the deviation parameter is essentially constant across many microns of image. By a happy accident, this method can be used, not only to correct for the helicity imparted by immersion lenses, but also to correct for buckling of the sample (up to a point).

Year:  2006        PMID: 16442733     DOI: 10.1016/j.ultramic.2005.12.002

Source DB:  PubMed          Journal:  Ultramicroscopy        ISSN: 0304-3991            Impact factor:   2.689


  4 in total

Review 1.  Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM.

Authors:  Robert M Glaeser; Dieter Typke; Peter C Tiemeijer; James Pulokas; Anchi Cheng
Journal:  J Struct Biol       Date:  2010-12-21       Impact factor: 2.867

Review 2.  Challenges and opportunities in cryo-EM single-particle analysis.

Authors:  Dmitry Lyumkis
Journal:  J Biol Chem       Date:  2019-02-25       Impact factor: 5.157

3.  New tools for automated high-resolution cryo-EM structure determination in RELION-3.

Authors:  Jasenko Zivanov; Takanori Nakane; Björn O Forsberg; Dari Kimanius; Wim Jh Hagen; Erik Lindahl; Sjors Hw Scheres
Journal:  Elife       Date:  2018-11-09       Impact factor: 8.140

Review 4.  Combining high throughput and high quality for cryo-electron microscopy data collection.

Authors:  Felix Weis; Wim J H Hagen
Journal:  Acta Crystallogr D Struct Biol       Date:  2020-07-27       Impact factor: 7.652

  4 in total

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