| Literature DB >> 1644204 |
D A Priestman1, S C Mistry, A L Kerbey, P J Randle.
Abstract
Rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP), a free PDH kinase readily separable from PDH complex and its intrinsic kinase, has been purified to apparent homogeneity from liver mitochondria of fed and 48-h starved rats. On SDS-PAGE an apparently single band of M(r) 45 kDa was obtained. N-Terminal amino acid sequence analyses (8-10 cycles) confirmed the presence of a single peptide in each case. The specific activity of the purified KAP from 48-h starved rats (14,413 U/mg protein) was 4.5-fold greater than that from fed rats.Entities:
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Year: 1992 PMID： 1644204 DOI： 10.1016/0014-5793(92)81056-r
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124