AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4+/-3.12%) compared to the control group (3.12+/-0.13%, P < 0.01). CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.
AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the humangastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the humangastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the humangastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4+/-3.12%) compared to the control group (3.12+/-0.13%, P < 0.01). CONCLUSION:Human mutant p27 can induce apoptosis of the humangastric cancer cells in vitro.
Authors: Louise Natalie Winteringham; Simon Kobelke; James Howard Williams; Evan Ingley; Svend Peter Klinken Journal: Oncogene Date: 2004-06-24 Impact factor: 9.867