PURPOSE: To assess if molecular detection of minimal disseminated disease by real-time reverse transcription and polymerase chain reaction (RT-PCR) could contribute to a better treatment stratification in patients with rhabdomyosarcoma (RMS). METHODS: Relative quantification of the tumor-mRNA present in serial samples of bone marrow (BM) and peripheral blood (PB) from 16 patients with RMS (7 alveolar and 9 embryonal) was performed by a real-time RT-PCR assay. Expression of MyoD1 and acetylcholine receptor (AChR) was analyzed in all samples, along with PAX3/7-FKHR in samples from alveolar tumors. RESULTS: A good correlation was found between the expression of PAX3/7-FKHR and AChR, while MyoD1 was more sensitive but less specific. In this study, patients with positive PB at the end of treatment showed a poorer prognosis than patients with negative PB. Moreover, in this patient cohort, metastatic relapses were preceded by the detection of microcirculating disease in all cases. CONCLUSION: The detection of minimal circulating and micrometastatic disease by real-time RT-PCR, based on the expression of multiple genes, yields highly reproducible results. Patients with positive PB after treatment show poorer survival than patients without microcirculating disease.
PURPOSE: To assess if molecular detection of minimal disseminated disease by real-time reverse transcription and polymerase chain reaction (RT-PCR) could contribute to a better treatment stratification in patients with rhabdomyosarcoma (RMS). METHODS: Relative quantification of the tumor-mRNA present in serial samples of bone marrow (BM) and peripheral blood (PB) from 16 patients with RMS (7 alveolar and 9 embryonal) was performed by a real-time RT-PCR assay. Expression of MyoD1 and acetylcholine receptor (AChR) was analyzed in all samples, along with PAX3/7-FKHR in samples from alveolar tumors. RESULTS: A good correlation was found between the expression of PAX3/7-FKHR and AChR, while MyoD1 was more sensitive but less specific. In this study, patients with positive PB at the end of treatment showed a poorer prognosis than patients with negative PB. Moreover, in this patient cohort, metastatic relapses were preceded by the detection of microcirculating disease in all cases. CONCLUSION: The detection of minimal circulating and micrometastatic disease by real-time RT-PCR, based on the expression of multiple genes, yields highly reproducible results. Patients with positive PB after treatment show poorer survival than patients without microcirculating disease.
Authors: S Gattenloehner; B Dockhorn-Dworniczak; I Leuschner; A Vincent; H K Müller-Hermelink; A Marx Journal: J Mol Diagn Date: 1999-11 Impact factor: 5.568
Authors: S Gattenloehner; A Vincent; I Leuschner; S Tzartos; H K Müller-Hermelink; T Kirchner; A Marx Journal: Am J Pathol Date: 1998-02 Impact factor: 4.307
Authors: W A Newton; E A Gehan; B L Webber; H B Marsden; A J van Unnik; A B Hamoudi; M G Tsokos; H Shimada; D Harms; D Schmidt Journal: Cancer Date: 1995-09-15 Impact factor: 6.860
Authors: W E Loh; H J Scrable; E Livanos; M J Arboleda; W K Cavenee; M Oshimura; B E Weissman Journal: Proc Natl Acad Sci U S A Date: 1992-03-01 Impact factor: 11.205
Authors: Lenka Krsková; Marcela Mrhalová; Irena Hilská; David Sumerauer; Eva Drahokoupilová; Peter Múdry; Roman Kodet Journal: Virchows Arch Date: 2010-04-20 Impact factor: 4.064
Authors: Ludmilla T Domingos Chinen; Celso A Lopes Mello; Emne Ali Abdallah; Luciana Mm Ocea; Marcilei E Buim; Natália M Breve; José Luiz Gasparini; Marcello F Fanelli; Patrizia Paterlini-Bréchot Journal: Onco Targets Ther Date: 2014-09-16 Impact factor: 4.147
Authors: K Beiske; S A Burchill; I Y Cheung; E Hiyama; R C Seeger; S L Cohn; A D J Pearson; K K Matthay Journal: Br J Cancer Date: 2009-04-28 Impact factor: 7.640
Authors: Marta Tellez-Gabriel; Hannah K Brown; Robin Young; Marie-Françoise Heymann; Dominique Heymann Journal: Front Oncol Date: 2016-09-07 Impact factor: 6.244