Literature DB >> 16430854

A Förster-resonance-energy transfer-based method for fluorescence detection of the protein redox state.

Sofya Kuznetsova1, Gerhild Zauner, Ralf Schmauder, Oleg A Mayboroda, André M Deelder, Thijs J Aartsma, Gerard W Canters.   

Abstract

A method for fluorescence detection of a protein's redox state based on resonance energy transfer from an attached fluorescence label to the prosthetic group of the redox protein is described and tested for proteins containing three types of prosthetic groups: a type-1 copper site (azurin, amicyanin, plastocyanin, and pseudoazurin), a heme group (cytochrome c550), and a flavin mononucleotide (flavodoxin). This method permits one to reliably distinguish between reduced and oxidized proteins and to perform potentiometric titrations at submicromolar concentrations.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16430854     DOI： 10.1016/j.ab.2005.11.036

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Ligand-induced monomerization of Allochromatium vinosum cytochrome c' studied using native mass spectrometry and fluorescence resonance energy transfer.

Authors:  Toon H Evers; Joost L J van Dongen; E W Meijer; Maarten Merkx
Journal:  J Biol Inorg Chem       Date:  2007-06-02       Impact factor: 3.358

2.  The enzyme mechanism of nitrite reductase studied at single-molecule level.

Authors:  Sofya Kuznetsova; Gerhild Zauner; Thijs J Aartsma; Hans Engelkamp; Nikos Hatzakis; Alan E Rowan; Roeland J M Nolte; Peter C M Christianen; Gerard W Canters
Journal:  Proc Natl Acad Sci U S A       Date:  2008-02-26       Impact factor: 11.205

Review 3.  Fundamentals and commercial aspects of nanobiosensors in point-of-care clinical diagnostics.

Authors:  Kuldeep Mahato; Pawan Kumar Maurya; Pranjal Chandra
Journal:  3 Biotech       Date:  2018-02-23       Impact factor: 2.406

4.  Fluorescence dequenching makes haem-free soluble guanylate cyclase detectable in living cells.

Authors:  Linda S Hoffmann; Peter M Schmidt; Yvonne Keim; Carsten Hoffmann; Harald H H W Schmidt; Johannes-Peter Stasch
Journal:  PLoS One       Date:  2011-08-17       Impact factor: 3.240

Review 5.  In Situ Spectroelectrochemical Investigations of Electrode-Confined Electron-Transferring Proteins and Redox Enzymes.

Authors:  Daniel H Murgida
Journal:  ACS Omega       Date:  2021-01-27

6.  Direct interrogation of DNA content distribution in nanoparticles by a novel microfluidics-based single-particle analysis.

Authors:  Cyrus W Beh; Deng Pan; Jason Lee; Xuan Jiang; Kelvin J Liu; Hai-Quan Mao; Tza-Huei Wang
Journal:  Nano Lett       Date:  2014-07-31       Impact factor: 11.189
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.