BACKGROUND: High-risk human papilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods. METHODS: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPoint). RESULTS: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques. CONCLUSIONS: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method.
BACKGROUND: High-risk humanpapilloma viruses (HPV) are reported to be significant independent risk factors for oral squamous cell carcinoma (OSCC). The prevalence of HPV in OSCC in a South African population sample was evaluated comparing three different HPV detection methods. METHODS: Tumour and adjacent morphologically normal oral mucosa of 59 resections of primary OSCC were evaluated for the presence of HPV using real-time polymerase chain reaction (PCR), conventional in situ hybridization (ISH), and a signal amplification ISH technique (Dako GenPoint). RESULTS: HPV18 DNA was detected in seven cases using real-time PCR. No positivity was found with the other two ISH techniques. CONCLUSIONS: We support the view that HPV is probably unimportant in the pathogenesis of OSCC and hypothesize HPV detection techniques as the main reason for the positive results in many studies. Real-time PCR was confirmed as the most sensitive technique, but researchers are urged to incorporate strict internal controls when using this detection method.
Authors: Cherie Paquette; Mark F Evans; Shabnum S Meer; Vanitha Rajendran; Christine S-C Adamson; Kumarasen Cooper Journal: Head Neck Pathol Date: 2013-12
Authors: Jerry Machado; Patricia P Reis; Tong Zhang; Colleen Simpson; Wei Xu; Bayardo Perez-Ordonez; David P Goldstein; Dale H Brown; Ralph W Gilbert; Patrick J Gullane; Jonathan C Irish; Suzanne Kamel-Reid Journal: Head Neck Oncol Date: 2010-03-12
Authors: Tania Rodríguez-Santamarta; Juan Pablo Rodrigo; Juana M García-Pedrero; Saúl Álvarez-Teijeiro; M Ángeles Villaronga; Laura Suárez-Fernández; Marta E Alvarez-Argüelles; Aurora Astudillo; Juan Carlos de Vicente Journal: Eur Arch Otorhinolaryngol Date: 2016-06-24 Impact factor: 2.503