AIMS: Immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH) are both commonly used assays for evaluation of HER-2/neu status in breast cancer. However, there is still no consensus on which method is most predictive of patient response to Herceptin. Recently, the automated cellular imaging system (ACIS) has been shown to improve the accuracy and reproducibility in scoring IHC. Our aim was to compare the results of HER-2/neu expression and gene amplification in the same patients by IHC using the ACIS system and by FISH. METHODS AND RESULTS: Two hundred and forty-seven breast cancer cases were studied. The concordance rate between IHC-ACIS (> or = 2.2) and FISH (> or = 2.0) was 94%. Fifteen patients were discordant; three had borderline FISH values and three had borderline IHC values. The other nine discordant cases consisted of five IHC-ACIS+, FISH- and six IHC-ACIS-, FISH+. HER-2/neu overexpression was more common in tumours that were high-grade, aneuploid, progesterone receptor and bcl-2 negative, with MIB-1 > 10%. CONCLUSION: HER-2/neu assessment by the ACIS is reliable, rapid and inexpensive, and correlates highly with results obtained by FISH.
AIMS: Immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH) are both commonly used assays for evaluation of HER-2/neu status in breast cancer. However, there is still no consensus on which method is most predictive of patient response to Herceptin. Recently, the automated cellular imaging system (ACIS) has been shown to improve the accuracy and reproducibility in scoring IHC. Our aim was to compare the results of HER-2/neu expression and gene amplification in the same patients by IHC using the ACIS system and by FISH. METHODS AND RESULTS: Two hundred and forty-seven breast cancer cases were studied. The concordance rate between IHC-ACIS (> or = 2.2) and FISH (> or = 2.0) was 94%. Fifteen patients were discordant; three had borderline FISH values and three had borderline IHC values. The other nine discordant cases consisted of five IHC-ACIS+, FISH- and six IHC-ACIS-, FISH+. HER-2/neu overexpression was more common in tumours that were high-grade, aneuploid, progesterone receptor and bcl-2 negative, with MIB-1 > 10%. CONCLUSION:HER-2/neu assessment by the ACIS is reliable, rapid and inexpensive, and correlates highly with results obtained by FISH.
Authors: Fritcher Emily G Barr; Amy C Degnim; Lynn C Hartmann; Derek C Radisky; Judy C Boughey; Stephanie S Anderson; Robert A Vierkant; Marlene H Frost; Daniel W Visscher; Carol Reynolds Journal: Cancer Prev Res (Phila) Date: 2011-01-05
Authors: Marta Santisteban; Carol Reynolds; Emily G Barr Fritcher; Marlene H Frost; Robert A Vierkant; Stephanie S Anderson; Amy C Degnim; Daniel W Visscher; V Shane Pankratz; Lynn C Hartmann Journal: Breast Cancer Res Treat Date: 2009-09-23 Impact factor: 4.872
Authors: Lynne Dobson; Catherine Conway; Alan Hanley; Alex Johnson; Sean Costello; Anthony O'Grady; Yvonne Connolly; Hilary Magee; Daniel O'Shea; Michael Jeffers; Elaine Kay Journal: Histopathology Date: 2010-06-24 Impact factor: 5.087
Authors: Andrew M Evens; Laurie H Sehn; Pedro Farinha; Beverly P Nelson; Adekunle Raji; Yi Lu; Adam Brakman; Vamsi Parimi; Jane N Winter; Paul T Schumacker; Randy D Gascoyne; Leo I Gordon Journal: J Clin Oncol Date: 2010-01-04 Impact factor: 44.544