| Literature DB >> 16428789 |
Sara Schesser Bartra1, Michael W Jackson, Julia A Ross, Gregory V Plano.
Abstract
A series of four large deletions that removed a total of ca. 36 kb of DNA from the ca. 70-kb Yersinia pestis pCD1 virulence plasmid were constructed using lambda Red-mediated recombination. Escherichia coli hha deletion mutants carrying the virulence plasmid with the deletions expressed a functional calcium-regulated type III secretion system. The E. coli hha/pCD1 system should facilitate molecular studies of the type III secretion process.Entities:
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Year: 2006 PMID: 16428789 PMCID: PMC1360333 DOI: 10.1128/IAI.74.2.1381-1386.2006
Source DB: PubMed Journal: Infect Immun ISSN: 0019-9567 Impact factor: 3.441