| Literature DB >> 16427712 |
Paul H M Savelkoul1, Arnold Catsburg, Sije Mulder, Ludo Oostendorp, Jurjen Schirm, Hans Wilke, Adri G M van der Zanden, Gerda T Noordhoek.
Abstract
The sensitivity and specificity to detect Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the IS6110 sequence and set 4 targeted a location 200 bp downstream on IS6110. Real Time PCR's with sets 1, 2 and 3 were carried out with co-amplification of a modified target as an internal amplification control. By testing identical DNA samples it was shown that small changes in primer and probe sequences result in differences in the performance of the assays, regarding analytical sensitivity and specificity.Entities:
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Year: 2006 PMID： 16427712 DOI： 10.1016/j.mimet.2005.12.003
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363