Literature DB >> 16426620

Fractionation of polyphenols in hawthorn into polymeric procyanidins, phenolic acids and flavonoids prior to high-performance liquid chromatographic analysis.

Ulla Svedström1, Heikki Vuorela, Risto Kostiainen, Into Laakso, Raimo Hiltunen.   

Abstract

Polymeric procyanidins, phenolic carboxylic acids and flavonoids of hawthorn (Crataegus laevigata) were fractionated prior to HPLC analysis using column chromatography and solid-phase extraction (SPE). The flavonoid fraction also contained (-)-epicatechin. The three groups of phenolics, each with clearly different UV spectra, were examined by means of high-performance liquid chromatography-diode array detection (HPLC-DAD) analysis. The average repeatability of the method (RSD) was in the range of 8-13% for chlorogenic acid, (-)-epicatechin and hyperoside. The polymeric procyanidins of hawthorn flowers consisted mainly of (-)-epicatechin subunits, and their mean degree of polymerization (DP) was 22.2. The HPLC methods developed can be used for the qualitative and quantitative analysis of different phenolic compounds in hawthorn plant material and their extracts.

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Year:  2006        PMID： 16426620     DOI： 10.1016/j.chroma.2005.12.080

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  7 in total

1.  In Vitro Anti-cancer Effect of Crataegus oxyacantha Berry Extract on Hormone Receptor Positive and Triple Negative Breast Cancers via Regulation of Canonical Wnt Signaling Pathway.

Authors:  Salini Kombiyil; Niranjali Devaraj Sivasithamparam
Journal:  Appl Biochem Biotechnol       Date:  2022-03-09       Impact factor: 2.926

2.  Preparative Separation of Procyanidins from Cocoa Polyphenolic Extract: Comparative Study of Different Fractionation Techniques.

Authors:  Said Toro-Uribe; Miguel Herrero; Eric A Decker; Luis Javier López-Giraldo; Elena Ibáñez
Journal:  Molecules       Date:  2020-06-19       Impact factor: 4.411

Review 3.  Polyphenolic profile and biological activity of Chinese hawthorn (Crataegus pinnatifida BUNGE) fruits.

Authors:  Tunde Jurikova; Jiri Sochor; Otakar Rop; Jiri Mlcek; Stefan Balla; Ladislav Szekeres; Vojtech Adam; Rene Kizek
Journal:  Molecules       Date:  2012-12-06       Impact factor: 4.411

4.  Procyanidin C1 from Viola odorata L. inhibits Na+,K+-ATPase.

Authors:  Tomas Heger; Marek Zatloukal; Martin Kubala; Miroslav Strnad; Jiri Gruz
Journal:  Sci Rep       Date:  2022-04-29       Impact factor: 4.996

5.  Determination of Flavonoids and Resveratrol in Wine by Turbulent-Flow Chromatography-LC-MS.

Authors:  Michele Antoniuk Presta; Ben Bruyneel; Renato Zanella; Jeroen Kool; Johannes G Krabbe; Henk Lingeman
Journal:  Chromatographia       Date:  2009-05-26       Impact factor: 2.044

6.  Plausible computational insights and new atomic-level perspective of epicathechin gallate from Crataegus oxycantha extract in preventing caspase 3 activation in conditions like post-myocardial infarction.

Authors:  Arul Salomee Kamalabai Ravindran; Sivaramakrishnan Venkatabalasubramanian; Ranjani Manickam; Muthuswamy Anusuyadevi; Jayachandran K Swaminathan
Journal:  J Biomol Struct Dyn       Date:  2020-11-17       Impact factor: 5.235

7.  Effects of simulated digestion on the phenolic composition and antioxidant activity of different cultivars of lychee pericarp.

Authors:  Qingzhu Zeng; Zhuohui Xu; Mingrui Dai; Xuejiao Cao; Xiong Xiong; Shan He; Yang Yuan; Mingwei Zhang; Lihong Dong; Ruifen Zhang; Dongxiao Su
Journal:  BMC Chem       Date:  2019-03-09
  7 in total

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