Literature DB >> 16424604

Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.).

Marcin Filipecki1, Zhimin Yin, Anita Wiśniewska, Mieczysław Smiech, Robert Malinowski, Stefan Malepszy.   

Abstract

Somaclonal variation commonly occurs during in vitro plant regeneration and may introduce unintended changes in numerous plant characters. In order to assess the range of tissue-culture-responsive changes on the biochemical level, the metabolic profiles of diploid and tetraploid cucumber R1 plants regenerated from leaf-derived callus were determined. Gas chromatography and mass spectrometry were used for monitoring of 48 metabolites and many significant changes were found in metabolic profiles of these plants as compared to a seed-derived control. Most of the changes were common to diploids and tetraploids and were effects of tissue culture. However, tetraploids showed quantitative changes in 14 metabolites, as compared to regenerated diploids. These changes include increases in serine, glucose-6P, fructose-6P, oleic acid and shikimic acid levels. Basing on this study we conclude that the variation in metabolic profiles does not correlate directly with the range of genome changes in tetraploids.

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Year:  2006        PMID： 16424604     DOI： 10.1007/BF03194594

Source DB:  PubMed          Journal:  J Appl Genet        ISSN： 1234-1983            Impact factor:   3.240


  8 in total

Review 1.  The biosynthesis of shikimate metabolites.

Authors:  Andrew R Knaggs
Journal:  Nat Prod Rep       Date:  2003-02       Impact factor: 13.423

Review 2.  Novel patterns of gene expression in polyploid plants.

Authors:  Keith L Adams; Jonathan F Wendel
Journal:  Trends Genet       Date:  2005-10       Impact factor: 11.639

3.  Somaclonal variation - a novel source of variability from cell cultures for plant improvement.

Authors:  P J Larkin; W R Scowcroft
Journal:  Theor Appl Genet       Date:  1981-10       Impact factor: 5.699

4.  Tissue culture-induced DNA methylation polymorphisms in repetitive DNA of tomato calli and regenerated plants.

Authors:  M J Smulders; W Rus-Kortekaas; B Vosman
Journal:  Theor Appl Genet       Date:  1995-12       Impact factor: 5.699

Review 5.  Genetic instability of plant tissue cultures: breakdown of normal controls.

Authors:  R L Phillips; S M Kaeppler; P Olhoft
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-07       Impact factor: 11.205

6.  Tissue culture-induced DNA methylation variation in maize.

Authors:  S M Kaeppler; R L Phillips
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

7.  Metabolite profiling for plant functional genomics.

Authors:  O Fiehn; J Kopka; P Dörmann; T Altmann; R N Trethewey; L Willmitzer
Journal:  Nat Biotechnol       Date:  2000-11       Impact factor: 54.908

8.  Silencing in Arabidopsis T-DNA transformants: the predominant role of a gene-specific RNA sensing mechanism versus position effects.

Authors:  Daniel Schubert; Berthold Lechtenberg; Alexandra Forsbach; Mario Gils; Sultan Bahadur; Renate Schmidt
Journal:  Plant Cell       Date:  2004-09-14       Impact factor: 11.277
  8 in total
  2 in total

1.  Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn.

Authors:  Alexander Betekhtin; Magdalena Rojek; Joanna Jaskowiak; Anna Milewska-Hendel; Jolanta Kwasniewska; Yulia Kostyukova; Ewa Kurczynska; Natalya Rumyantseva; Robert Hasterok
Journal:  PLoS One       Date:  2017-03-09       Impact factor: 3.240

2.  Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species.

Authors:  Hagai Cohen; Aaron Fait; Noemi Tel-Zur
Journal:  BMC Plant Biol       Date:  2013-11-04       Impact factor: 4.215
  2 in total

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