Literature DB >> 16423686

Detection of cdtA, cdtB, and cdtC genes in Campylobacter jejuni by multiplex PCR.

Irati Martínez1, Estibaliz Mateo, Estibaliz Churruca, Cecilia Girbau, Rodrigo Alonso, Aurora Fernández-Astorga.   

Abstract

A multiplex PCR was developed for simultaneous detection of the cytolethal distending toxin (cdt) genes of Campylobacter jejuni. Three primer pairs targeting each one of the cdtA, cdtB and cdtC genes were designed and combined in the same PCR reaction. The assay was evaluated with 100 C. jejuni strains recovered from humans and animals and it was found to be rapid and specific. Two isolates presented several deletions affecting both cdtA and cdtB genes. High prevalence (98%) of the three cdt genes was found among isolates of different geographic origins.

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Year:  2005        PMID： 16423686     DOI： 10.1016/j.ijmm.2005.08.003

Source DB:  PubMed          Journal:  Int J Med Microbiol        ISSN： 1438-4221            Impact factor:   3.473


  9 in total

1.  Chronic exposure to the cytolethal distending toxins of Gram-negative bacteria promotes genomic instability and altered DNA damage response.

Authors:  Riccardo Guidi; Lina Guerra; Laura Levi; Bo Stenerlöw; James G Fox; Christine Josenhans; Maria G Masucci; Teresa Frisan
Journal:  Cell Microbiol       Date:  2012-11-01       Impact factor: 3.715

2.  Molecular typing and cdt genes prevalence of Campylobacter jejuni isolates from various sources.

Authors:  Arzu Findik; Tuba Ica; Ertan Emek Onuk; Duygu Percin; Tahsin Onur Kevenk; Alper Ciftci
Journal:  Trop Anim Health Prod       Date:  2010-11-23       Impact factor: 1.559

3.  Agents of Campylobacteriosis in Different Meat Matrices in Brazil.

Authors:  Micaela Guidotti Takeuchi; Roberta Torres de Melo; Carolyne Ferreira Dumont; Jéssica Laura Miranda Peixoto; Gabriella Rayane Aparecida Ferreira; Mariana Comassio Chueiri; Jocasta Rodrigues Iasbeck; Marcela Franco Timóteo; Bárbara de Araújo Brum; Daise Aparecida Rossi
Journal:  Int J Environ Res Public Health       Date:  2022-05-17       Impact factor: 4.614

4.  Participation of the Cytoskeletal and Lysosomal Compartments in Campylobacter jejuni Invasion of Caco-2 cells, the Cellular Response by Morphometric Analysis and the Presence of Cytokine and Chemokine Transcripts.

Authors:  B B Fonseca; I L Santos; D A Rossi; R T Melo; T G Araújo; C U Vieira; E P Mendonça; M E Beletti
Journal:  Indian J Microbiol       Date:  2012-11-11       Impact factor: 2.461

Review 5.  Human campylobacteriosis: A public health concern of global importance.

Authors:  Aboi Igwaran; Anthony Ifeanyi Okoh
Journal:  Heliyon       Date:  2019-11-14

6.  Genetic Diversity of Campylobacter jejuni Isolated From Avian and Human Sources in Egypt.

Authors:  Marwa I Abd El-Hamid; Norhan K Abd El-Aziz; Mohamed Samir; El-Sayed Y El-Naenaeey; Etab M Abo Remela; Rasha A Mosbah; Mahmoud M Bendary
Journal:  Front Microbiol       Date:  2019-10-18       Impact factor: 5.640

7.  Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses.

Authors:  Yuli Melisa Sierra-Arguello; Gustavo Perdoncini; Laura Beatriz Rodrigues; Luciana Ruschel Dos Santos; Karen Apellanis Borges; Thales Quedi Furian; Carlos Tadeu Pippi Salle; Hamilton Luiz de Souza Moraes; Marcos José Pereira Gomes; Vladimir Pinheiro do Nascimento
Journal:  Sci Rep       Date:  2021-02-25       Impact factor: 4.379

8.  Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil.

Authors:  Aline Feola de Carvalho; Daniela Martins da Silva; Sergio Santos Azevedo; Rosa Maria Piatti; Margareth Elide Genovez; Eliana Scarcelli
Journal:  Braz J Microbiol       Date:  2014-01-15       Impact factor: 2.476

9.  Detection, identification and quantification of Campylobacter jejuni, coli and lari in food matrices all at once using multiplex qPCR.

Authors:  Lucie Vondrakova; Jarmila Pazlarova; Katerina Demnerova
Journal:  Gut Pathog       Date:  2014-05-09       Impact factor: 4.181
  9 in total

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