| Literature DB >> 16422303 |
Raquel Cordeiro Theodoro1, João Manuel Grisi Candeias, João Pessoa Araújo, Sandra De Moraes Gimenes Bosco, Severino Assis Da Graça Macoris, Luis Otavio Junior Padula, Marcello Franco, Eduardo Bagagli.
Abstract
The objective of the present study was to evaluate different techniques for the detection of Paracoccidioides brasiliensis in soil, e.g., culture, animal inoculation and specific DNA amplification by Nested PCR. We designed species-specific inner primers derived from rDNA regions (ITS, 5.8S gene) and found their sensitivity to be higher than culture and animal inoculation. In addition, the sensitivity of these primers was higher than p27-gene primers developed for detection of P. brasiliensis in soil in a previous study. DNA from P. brasiliensis was detected in soil artificially seeded with the fungus (positive soil control) and from environmental samples collected in an armadillo burrow.Entities:
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Year: 2005 PMID： 16422303 DOI： 10.1080/13693780500129418
Source DB: PubMed Journal: Med Mycol ISSN： 1369-3786 Impact factor: 4.076