Literature DB >> 1641340

Two promoter elements are necessary and sufficient for expression of the sea urchin U1 snRNA gene.

B J Wendelburg1, W F Marzluff.   

Abstract

The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between -318 and -300 and a proximal sequence element (PSE) centered at -55. Removal or alteration of other sequences conserved in different sea urchin snRNA U1 genes, including deletion of all sequence between -90 and -273, did not affect the expression. Sequences around the start site were not required for expression. Deletion of nucleotides between the PSE and the start site resulted in initiation inside the U1 coding region, suggesting that the PSE determines the start site of transcription. There is no obvious similarity between the sequences required for the sea urchin U1 snRNA expression and the sequences required for the expression of other sea urchin snRNAs.

Entities:  

Mesh:

Substances:

Year:  1992        PMID: 1641340      PMCID: PMC334027          DOI: 10.1093/nar/20.14.3743

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  30 in total

1.  Nucleotide sequence of the mouse U7 snRNA gene.

Authors:  S C Phillips; P C Turner
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

2.  Characterization of two developmentally regulated sea urchin U2 small nuclear RNA promoters: a common required TATA sequence and independent proximal and distal elements.

Authors:  B Stefanovic; W F Marzluff
Journal:  Mol Cell Biol       Date:  1992-02       Impact factor: 4.272

3.  Organization and transient expression of the gene for human U11 snRNA.

Authors:  C Suter-Crazzolara; W Keller
Journal:  Gene Expr       Date:  1991-05

4.  A conserved region in the sea urchin U1 snRNA promoter interacts with a developmentally regulated factor.

Authors:  K A Stevenson; J C Yu; W F Marzluff
Journal:  Nucleic Acids Res       Date:  1992-01-25       Impact factor: 16.971

5.  Isolation of an active gene and of two pseudogenes for mouse U7 small nuclear RNA.

Authors:  A Gruber; D Soldati; M Burri; D Schümperli
Journal:  Biochim Biophys Acta       Date:  1991-01-17

6.  Structure and expression of a second sea urchin U1 RNA gene repeat.

Authors:  J C Yu; M A Nash; C Santiago; W F Marzluff
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

7.  A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.

Authors:  P Carbon; S Murgo; J P Ebel; A Krol; G Tebb; L W Mattaj
Journal:  Cell       Date:  1987-10-09       Impact factor: 41.582

8.  Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements.

Authors:  M Tanaka; U Grossniklaus; W Herr; N Hernandez
Journal:  Genes Dev       Date:  1988-12       Impact factor: 11.361

9.  Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.

Authors:  S Henikoff
Journal:  Gene       Date:  1984-06       Impact factor: 3.688

10.  Human U2 small nuclear RNA genes contain an upstream enhancer.

Authors:  M Mangin; M Ares; A M Weiner
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598

View more
  11 in total

1.  Similarities and differences in the conformation of protein-DNA complexes at the U1 and U6 snRNA gene promoters.

Authors:  S B Hardin; C J Ortler; K J McNamara-Schroeder; W E Stumph
Journal:  Nucleic Acids Res       Date:  2000-07-15       Impact factor: 16.971

2.  Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements.

Authors:  D Zhou; S M Lobo-Ruppert
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

3.  The proximal sequence element (PSE) plays a major role in establishing the RNA polymerase specificity of Drosophila U-snRNA genes.

Authors:  R C Jensen; Y Wang; S B Hardin; W E Stumph
Journal:  Nucleic Acids Res       Date:  1998-01-15       Impact factor: 16.971

4.  Identification and topological arrangement of Drosophila proximal sequence element (PSE)-binding protein subunits that contact the PSEs of U1 and U6 small nuclear RNA genes.

Authors:  Y Wang; W E Stumph
Journal:  Mol Cell Biol       Date:  1998-03       Impact factor: 4.272

5.  The proximal element is capable of determining proper temporal expression of the embryonic sea urchin U2 snRNA gene.

Authors:  B Stefanovic; W F Marzluff
Journal:  Gene Expr       Date:  1994

6.  Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box.

Authors:  J M Li; R A Parsons; W F Marzluff
Journal:  Mol Cell Biol       Date:  1994-03       Impact factor: 4.272

7.  Small nuclear RNA genes transcribed by either RNA polymerase II or RNA polymerase III in monocot plants share three promoter elements and use a strategy to regulate gene expression different from that used by their dicot plant counterparts.

Authors:  S Connelly; C Marshallsay; D Leader; J W Brown; W Filipowicz
Journal:  Mol Cell Biol       Date:  1994-09       Impact factor: 4.272

8.  Common factors direct transcription through the proximal sequence elements (PSEs) of the embryonic sea urchin U1, U2, and U6 genes despite minimal similarity among the PSEs.

Authors:  J M Li; R P Haberman; W F Marzluff
Journal:  Mol Cell Biol       Date:  1996-03       Impact factor: 4.272

9.  RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements.

Authors:  A Fantoni; A O Dare; C Tschudi
Journal:  Mol Cell Biol       Date:  1994-03       Impact factor: 4.272

Review 10.  The assembly of a spliceosomal small nuclear ribonucleoprotein particle.

Authors:  Snehal Bhikhu Patel; Michel Bellini
Journal:  Nucleic Acids Res       Date:  2008-10-14       Impact factor: 16.971

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.